“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”

Registration ID: IJNRD_202799

Published ID: IJNRDTH00070

: Click Here to Get

Dr Amol Yadavrao Ghodke, Vishal Giri, Dr B N Poul, Dr S S Patil , Dr C V Panchal

RP-HPLC, Torsemide, Zorbax C18, specificity, linearity, precision, accuracy.

A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Methylcobalamin, alpha lipoic acid, pyridoxine and folic acid by RP-HPLC in bulk and marketed dosage form”. The proposed RP-HPLC method was carried out on 250 mm x 4.6 mm, 5um column with mobile 0.1% o-phosphoric acid: Methanol (50:50). The retention time of methylcobalamin, alpha lipoic acid, pyridoxine and folic acid was found at 2.33 ±0.2min, 3.39 ±0.2min, 5.27 ±0.2min, 6.91 ±0.2min respectively. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 ug/mL and correlation coefficient (r2) was found within the range. The mean % recovery for Torsemide was found to be 99.80.

Published Paper   E-Certificate

Dr Amol Yadavrao Ghodke, Vishal Giri, Dr B N Poul, Dr S S Patil , & Dr C V Panchal (August-2023). “DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(8), 526-610. https://ijnrd.org/papers/IJNRDTH00070.pdf

Volume 8 Issue 8, August-2023

Pages : 526-610

Paper Reg. ID: IJNRD_202799

Published Paper Id: IJNRDTH00070

Downloads: 000122074

Research Area: Pharmacy

Author Type: Indian Author

Country: Andur, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRDTH00070.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRDTH00070

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

UGC CARE JOURNAL PUBLICATION | ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave | IJNRD.ORG | IJNRD.COM | IJPUB.ORG

© 2025 — Authors hold the copyright of this article. This work is licensed under a Creative Commons Attribution 4.0 International License. and The Open Definition.

You are free to share, adapt, and redistribute the material, provided proper credit is given to the original author. 🛡️ Disclaimer: The content, data, and findings in this article are based on the authors’ research and have been peer-reviewed for academic purposes only. Readers are advised to verify all information before practical or commercial use. The journal and its editorial board are not liable for any errors, losses, or consequences arising from its use.

Call For Paper - Volume 10 | Issue 11 | November 2025

IJNRD is a Scholarly Open Access, Peer-Reviewed, Refereed, and UGC CARE Journal Publication with a High Impact Factor of 8.76 (calculated by Google Scholar & Semantic Scholar | AI-Powered Research Tool). It is a Multidisciplinary, Monthly, Low-Cost, and Transparent Peer Review Journal Publication that adheres to the UGC CARE 2025 Peer-Reviewed Journal Policy and aligns with Scopus Journal Publication standards to ensure the highest level of research quality and credibility.

IJNRD offers comprehensive Journal Publication Services including indexing in all major databases and metadata repositories, Digital Object Identifier (Crossref DOI) assignment for each published article with additional fees, citation generation tools, and full Open Access visibility to enhance global research reach and citation impact.

The INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to advance applied, theoretical, and experimental research across diverse academic and professional fields. The journal promotes global knowledge exchange among researchers, developers, academicians, engineers, and practitioners, serving as a trusted platform for innovative, peer-reviewed journal publication and scientific collaboration.

Indexing Coverage: Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar (AI-Powered Research Tool), Microsoft Academic, Academia.edu, arXiv.org, ResearchGate, CiteSeerX, ResearcherID (Thomson Reuters), Mendeley, DocStoc, ISSUU, Scribd, and many other recognized academic repositories.

How to submit the paper?

By Our website

Click Here to Submit Paper Online

By Mail

Send your paper to editor@ijnrd.org

You can now publish your research in IJNRD. IJNRD is a Transparent Peer-Reviewed Open Access Journal Publication (Refereed Journal), aligning with New UGC and UGC CARE recommendations.

For more details, refer to the official notice: UGC Public Notice

Submit Paper Online

Important Dates for Current issue

Paper Submission Open For: November 2025

Current Issue: Volume 10 | Issue 11 | November 2025

Impact Factor: 8.76

Last Date for Paper Submission: Till 30-Nov-2025

Notification of Review Result: Within 1-2 Days after Submitting paper.

Publication of Paper: Within 01-02 Days after Submititng documents.

Frequency: Monthly (12 issue Annually).

Journal Type: IJNRD is an International Peer-reviewed, Refereed, and Open Access Journal with Transparent Peer Review as per the new UGC CARE 2025 guidelines, offering low-cost multidisciplinary publication with Crossref DOI and global indexing.

Subject Category: Research Area

Call for Paper: More Details

Approval, Licenses and Indexing: More Details