Method Development and validation for Simultaneous Estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate Opthalmic Suspension by RP-HPLC

Registration ID: IJNRD_308352

Published ID: IJNRD2507163

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Sanjay Kumar Karan, Subhasmita Subhadarshinee, Soumya Ranjan Pradhan

Moxifloxacin Hydrochloride, Loteprednol Etabonate, UV-Visible Spectrophotometer, Validation Methods.

For the estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate in pharmaceutical tablet dosage forms an accurate, simple, rapid, efficient, highly sensitive, precise Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed and validated. Hypersil Gold column (250mm x 4.6mm; 5μ) was used in gradient mode to develop the RP-HPLC method. The mobile phase A (Buffer) contained potassium dihydrogen ortho phosphate pH adjusted to 3.0 with ortho phosphoric acid. The mobile phase B (Organic phase) contained a 60:40 v/v ratio of acetonitrile and methanol. The flow rate was 1.0ml/min, and the effluent was measured at 274nm UV wavelength using a PharmaSpec UV-1700 UV-Vis spectrophotometer. The retention time of Moxifloxacin was 9.541 and Loteprednol was 14.210 minutes. According to ICH guidelines, the method was validated with respect to system suitability, specificity, precision, accuracy, linearity and range, robustness and ruggedness. The linearity with range of Moxifloxacin Hydrochloride was found to be 0.03015 mg/ml to 0.09045 mg/ml, with a correlation coefficient of 0.9999. The linearity with range of Loteprednol Etabonate was found to be 0.05015 mg/ml to 0.15045 mg/ml, with a correlation coefficient of 0.9999. The developed method was successfully used to quantify the bulk and active pharmaceutical ingredient content of tablet dosage form.

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Sanjay Kumar Karan, Subhasmita Subhadarshinee, & Soumya Ranjan Pradhan (July-2025). Method Development and validation for Simultaneous Estimation of Moxifloxacin Hydrochloride and Loteprednol Etabonate Opthalmic Suspension by RP-HPLC. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 10(7), b620-b633. https://ijnrd.org/papers/IJNRD2507163.pdf

Volume 10 Issue 7, July-2025

Pages : b620-b633

Paper Reg. ID: IJNRD_308352

Published Paper Id: IJNRD2507163

Downloads: 000121995

Research Area: Pharmacy All

Author Type: Indian Author

Country: Mayurbhanj, Odisha, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2507163.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2507163

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