Computational, biochemical and genomic analyses for a novel gene on human chromosome 12, C12ORF24 and structural analyses of its cognate protein, FAM216A.

Computational, biochemical and genomic analyses for a novel gene on human chromosome 12, C12ORF24 and structural analyses of its cognate protein, FAM216A.

Registration ID: IJNRD_300246

Published ID: IJNRD2409074

DOI: http://doi.one/10.1729/Journal.41529

Vishnupriya J

Chromosome 12 open reading frame 24(C12ORF24), Family with sequence similarity 216, member A(FAM216A),Basic Local Alignment Search Tool for -Nucleotide and –Protein(BLAST-N&-P),GPN-loop gtpase 3(GPN3),Expert Protein Analysis System(ExPASy), Search Tool for the Retrieval of Interacting Genes/Proteins (STRING db),Signal peptide site(Sp site).

The Human Genome Project was initiated to completely sequence our genome there still remain “uncharacterized crucial genes” in different chromosomes , under genetic changes lead to cancer prone diseases, solid tumors, leukemia, etc. This study represents a brief analysis particularly on chromosome 12 Open Reading Frame 24. The gene named as C12ORF24 and its novel protein as FAM216A, which is at the cytogenetic location q24.11.The C12ORF24 gene sequence retrived from the NCBI database and the BLAST (-N &-P) analysis performed within the species and also between the ortholog gene species. BLASTN result shows 100% nucleotide sequence similarity of Human gene C12ORF24 with the Human GPN3 transcript (reverse) and the genomic sequence of alternate assembly of Human C1 1.1. And shows 77% similarity over the species rat (C12) and mouse (C5) containing homolog gene. Likewise the amino acid sequence of human C12ORF24 compared with that of rat and mice showed 68% similarity in rat and 85% similarity in mice. So humans and mice , two species separated by more than million years of evolutionary history, have similar genes. Further Human gene study about the exon/intron organization revealed that three species containing homolog gene C12ORF24 has 7 exons and 6 introns and TSS, Poly(A) signal site, UTR’s(5’&3’), epigenetics -were annotated using the respective public database tools. The cognate protein (FAM216A) study revealed about its post –translational modification sites especially phosphorylation which determines its functional state of activity via ExPASy; its primary and secondary structure was predicted and compared with that of rat and mice having homolog protein. Tissue distribution inferred from the whole human proteome project also revealed a prominent existence of FAM216A protein in brain and testis, thereby connecting psychiatric and hormone related functions. The STRING db extensive protein – protein interactions results has shown that FAM216A protein interacts with Ubiquitin, suggesting that its action may be pivotal in determining brain function(s).This pivotal information will certainly increase our future knowledge on this human gene and may lead to the development of novel treatment modalities in this genomic era.

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"Computational, biochemical and genomic analyses for a novel gene on human chromosome 12, C12ORF24 and structural analyses of its cognate protein, FAM216A.", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.9, Issue 9, page no.a662-a680, September-2024, Available :https://ijnrd.org/papers/IJNRD2409074.pdf

Volume 9 Issue 9, September-2024

Pages : a662-a680

Paper Reg. ID: IJNRD_300246

Published Paper Id: IJNRD2409074

Downloads: 000121159

Research Area: Life Sciences All

Country: chennai / thiruvalluvar district, Tamilnadhu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2409074.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2409074

DOI: http://doi.one/10.1729/Journal.41529

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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