Paper Title
Purification And Partial Characterization Of Anti-Microbial Peptides Produced By Bacillus licheniformis Isolated From Curd
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Registration ID: IJNRD_225599
Published ID: IJNRD2407418
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Keywords
Bacillus licheniformis, antimicrobial, bio-preservative, bacteriocin, gel filtration chromatography
Abstract
Bacillus licheniformis, a potential saprophytic, Gram positive, endospore forming bacteria, is considered to be a species that yields a broad range of antimicrobial compounds. Natural antimicrobials are an alternative to the chemical and artificially synthesized substances which poses a threat to the health and well-being of living things. In the food industry, bacteriocins and other antimicrobial compounds derived from B. licheniformis are regarded as bio preservatives in preservation of food items. Potential bacterial species that produces these compounds can be screened from various sources like fermented foods, soil, water, plants, etc. The current study focuses on the purification of antimicrobial substances produced by bacterial species isolated from curd. The antimicrobial substances can be isolated from the cell free supernatant obtained by centrifugation of the bacterial culture broth. The bacterial species that produces these compounds can be identified by 16 SrRNA sequencing after carrying out the anti-bacterial activity testing. Further purification of the antimicrobial substances can be carried out by membrane filtration, ammonium sulfate precipitation, gel filtration chromatography, etc. The confirmation of protein in the purified fraction can be identified and confirmed by Lowry’s method of protein estimation and SDS PAGE. The inhibition of growth of Escherichia coli and Listeria monocytogenes showed that the purified proteins from Bacillus licheniformis can serve as natural food preservatives after safety testing. It may also be employed as biopreservatives in cosmetics and pharmaceuticals and used to control plant pathogens in agriculture. KEYWORDS : Bacillus licheniformis, antimicrobial, bio-preservative, bacteriocin, gel filtration chromatography
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How To Cite (APA)
Rose Mary Sunny , R. Arthi, & M. Shanmugavadivu (July-2024). Purification And Partial Characterization Of Anti-Microbial Peptides Produced By Bacillus licheniformis Isolated From Curd. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(7), f177-f184. https://ijnrd.org/papers/IJNRD2407418.pdf
Issue
Volume 9 Issue 7, July-2024
Pages : f177-f184
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Paper Reg. ID: IJNRD_225599
Published Paper Id: IJNRD2407418
Research Area: Life SciencesÂ
Author Type: Indian Author
Country: Thrissur , Kerala, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2407418.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2407418
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