DNA BARCODE AND MOLECULAR CONSERVATION OF SELECTED MEDICINAL PLANT SPECIES FROM KANNIYAKUMARI DISTRICT, TAMILNADU

Registration ID: IJNRD_224748

Published ID: IJNRD2407089

DOI: http://doi.one/10.1729/Journal.40441

Dr.J.L.LEKSHMI , V.Sundara Prabha , Sreeja

DNA barcoding has proven to be one of the successful methods for the rapid identification of species, and thus conserving them. This method has been widely used in the different fields including Boral exploration. In spite of debates for using suitable gene for plant species, matK and rbcL are selected as the core barcodes for plants. The availability of molecular data as well as modern technologies in DNA sequencing have made DNA barcoding a popular process in many taxonomic studies. DNA barcoding is not a replacement to the traditional taxonomic classification but seen as a complement to traditional taxonomy and to accelerate the identification process. This study was carried out with the aim to generate DNA barcodes, from Calophylum inophylum and Couroupita guianesis. Flower specimens were collected for the DNA analysis. For the extraction of DNA barcodes, all laboratory procedures have been done in TICEL bio park phase - Ill, Yaazh Xenomics, Coimbatore. Sequence editing was carefully done using MUSCLE 3.7 software to each successfully generated barcode. After the editing of the sequences, the molecular identification and phylogenetic trees were constructed. Using rbct, matK and combination of both markers, two phylogenetic trees were constructed in this study using two different methods (Neighbor Joining and Maximum Likelihood). For the construction of phylogenetic trees, sequences obtained from the leaves samples and sequences downloaded from NCBI were used. The rbcL, matK sequences from collected samples correctly clustered together with the Genbank sequences representing the same genera. However, only very few of sequences clustered together with the Genbank sequences representing same species. Keywords: matK, DNA barcoding, Sequence and Medicinal plants

Published Paper   E-Certificate

Dr.J.L.LEKSHMI, V.Sundara Prabha, & Sreeja (July-2024). DNA BARCODE AND MOLECULAR CONSERVATION OF SELECTED MEDICINAL PLANT SPECIES FROM KANNIYAKUMARI DISTRICT, TAMILNADU. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(7), a348-a356. http://doi.one/10.1729/Journal.40441

Volume 9 Issue 7, July-2024

Pages : a348-a356

Paper Reg. ID: IJNRD_224748

Published Paper Id: IJNRD2407089

Downloads: 000122001

Research Area: Life Sciences 

Country: Nagercoil, tamil nadu, India

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Crossref DOI: http://doi.one/10.1729/Journal.40441

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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