Paper Title
EXTRACTION OF DNA FROM SOIL USING NANOPARTICLES BY MAGNETIC BIO-SEPARATION
Article Identifiers
Authors
ANJANA A PILLAI , SURYA S KRISHNAN
Keywords
DNA, magnetic bio-separation, metagenome, PCR amplification, rapid techniques, soil, superparamagnetic silica magnetite nanoparticles, restriction endonuclease digestion, SoilMaster DNA extraction kit.
Abstract
DNA extraction from soil is an emerging technology to understand the microbial diversity of an environment or an effective tool to study environmental microbiology. Soil DNA extraction using standard methods results in low quality and poor yield, making it unsuitable for polymerase chain reaction (PCR) analysis. Two strategies have been developed to improve DNA recovery regarding yield, purity and unbiased representation of microbial diversity. The first approach consists of directly extracting nucleic acids from soil through in situ cell lysis followed by DNA purification. The alternative approach is based on the separation of bacteria from the soil particles followed by cell lysis and then DNA purification. The protocol relies on the use of superparamagnetic silica magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from several soil samples. This article presents the methodologies relies on the isolation and purification of DNA from soil samples by using nanoparticles, to develop a rapid, simple and inexpensive protocol. The main contribution of this work lies on the DNA isolation from soil samples based on purity, yield, PCR suitability and restriction digestion. This study suggests that the methodology or protocol described in this report is cost-effective, less time consuming, rapid, high-yielding and good-quality DNA from soil. The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested. The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil samples, and is scalable and suitable for automation.
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How To Cite (APA)
ANJANA A PILLAI & SURYA S KRISHNAN (June-2024). EXTRACTION OF DNA FROM SOIL USING NANOPARTICLES BY MAGNETIC BIO-SEPARATION. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), e377-e389. https://ijnrd.org/papers/IJNRD2406435.pdf
Issue
Volume 9 Issue 6, June-2024
Pages : e377-e389
Other Publication Details
Paper Reg. ID: IJNRD_224229
Published Paper Id: IJNRD2406435
Downloads: 000121981
Research Area: Biological Science
Country: thiruvananthapuram, Kerala, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2406435.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406435
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition
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