EXTRACTION OF DNA FROM SOIL USING NANOPARTICLES BY MAGNETIC BIO-SEPARATION

Registration ID: IJNRD_224229

Published ID: IJNRD2406435

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ANJANA A PILLAI, SURYA S KRISHNAN

DNA, magnetic bio-separation, metagenome, PCR amplification, rapid techniques, soil, superparamagnetic silica magnetite nanoparticles, restriction endonuclease digestion, SoilMaster DNA extraction kit.

DNA extraction from soil is an emerging technology to understand the microbial diversity of an environment or an effective tool to study environmental microbiology. Soil DNA extraction using standard methods results in low quality and poor yield, making it unsuitable for polymerase chain reaction (PCR) analysis. Two strategies have been developed to improve DNA recovery regarding yield, purity and unbiased representation of microbial diversity. The first approach consists of directly extracting nucleic acids from soil through in situ cell lysis followed by DNA purification. The alternative approach is based on the separation of bacteria from the soil particles followed by cell lysis and then DNA purification. The protocol relies on the use of superparamagnetic silica magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from several soil samples. This article presents the methodologies relies on the isolation and purification of DNA from soil samples by using nanoparticles, to develop a rapid, simple and inexpensive protocol. The main contribution of this work lies on the DNA isolation from soil samples based on purity, yield, PCR suitability and restriction digestion. This study suggests that the methodology or protocol described in this report is cost-effective, less time consuming, rapid, high-yielding and good-quality DNA from soil. The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested. The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil samples, and is scalable and suitable for automation.

Published Paper   E-Certificate

ANJANA A PILLAI & SURYA S KRISHNAN (June-2024). EXTRACTION OF DNA FROM SOIL USING NANOPARTICLES BY MAGNETIC BIO-SEPARATION. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), e377-e389. https://ijnrd.org/papers/IJNRD2406435.pdf

Volume 9 Issue 6, June-2024

Pages : e377-e389

Paper Reg. ID: IJNRD_224229

Published Paper Id: IJNRD2406435

Downloads: 000122259

Research Area: Biological Science

Author Type: Indian Author

Country: thiruvananthapuram, Kerala, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406435.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406435

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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