Stability-Indicating HPTLC Method Development and Validation for analysis of Colchicine in the Bulk Drug and Tablet Dosage Forms

Registration ID: IJNRD_224076

Published ID: IJNRD2406367

DOI: Click Here to Get

Mr Shubham Ramchandra Khade , Ms. prerna Pardeshi , Ms. Alka Bhure , Mr. Shivraj Vilas Mane

Colchicine, HPTLC, Method Validation, LODOCO

Colchicine, a potent alkaloid employed in the treatment of gout and inflammatory disorders, necessitates a robust analytical method due to its inherent instability and propensity for degradation. This study presents the development and validation of a stability-indicating High-Performance Thin-Layer Chromatography (HPTLC) method for the quantitative determination of colchicine in pharmaceutical formulations. The chromatographic conditions were optimized using silica gel plates with a mobile phase consisting of methanol, ethyl acetate, and glacial acetic acid in a 2:7:1 ratio. This method effectively separated colchicine from its degradation products, ensuring specificity under stress conditions. Colchicine spots were visualized at a wavelength of 355 nm utilizing UV detection. Validation of the method was conducted in accordance with International Conference on Harmonization (ICH) guidelines. The method exhibited excellent linearity over the concentration range, with a correlation coefficient (r) of 0.99724. Precision studies demonstrated low relative standard deviation (% RSD), with values for intra-day and inter-day variations found to be less than 2%, thereby confirming the method's repeatability and reproducibility. Accuracy was assessed through recovery studies, which yielded results within acceptable limits. In conclusion, the validated HPTLC method provides an effective solution for the quantitative determination of colchicine, ensuring pharmaceutical quality and stability assessment in drug formulations. This method's ability to accurately and reliably quantify colchicine, even in the presence of its degradation products, underscores its utility in the pharmaceutical industry, particularly in the quality control and stability testing of colchicine-containing formulations.

Published Paper   E-Certificate

Mr Shubham Ramchandra Khade, Ms. prerna Pardeshi, Ms. Alka Bhure, & Mr. Shivraj Vilas Mane (June-2024). Stability-Indicating HPTLC Method Development and Validation for analysis of Colchicine in the Bulk Drug and Tablet Dosage Forms. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), d768-d775. https://ijnrd.org/papers/IJNRD2406367.pdf

Volume 9 Issue 6, June-2024

Pages : d768-d775

Paper Reg. ID: IJNRD_224076

Published Paper Id: IJNRD2406367

Downloads: 000122001

Research Area: Pharmacy

Country: Pune, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406367.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406367

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave | IJNRD.ORG | IJNRD.COM | IJPUB.ORG

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

Call For Paper - Volume 10 | Issue 10 | October 2025

IJNRD is a Scholarly Open Access, Peer-reviewed, and Refereed Journal with a High Impact Factor of 8.76 (calculated by Google Scholar & Semantic Scholar | AI-Powered Research Tool). It is a Multidisciplinary, Monthly, Low-Cost Journal that follows UGC CARE 2025 Peer-Reviewed Journal Policy norms, Scopus journal standards, and Transparent Peer Review practices to ensure quality and credibility. IJNRD provides indexing in all major databases & metadata repositories, a citation generator, and Digital Object Identifier (DOI) for every published article with full open-access visibility.

The INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to advance applied, theoretical, and experimental research across diverse fields. Its goal is to promote global scientific information exchange among researchers, developers, engineers, academicians, and practitioners. IJNRD serves as a platform where educators and professionals can share research evidence, models of best practice, and innovative ideas, contributing to academic growth and industry relevance.

Indexing Coverage includes Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar (AI-Powered Research Tool), Microsoft Academic, Academia.edu, arXiv.org, ResearchGate, CiteSeerX, ResearcherID (Thomson Reuters), Mendeley, DocStoc, ISSUU, Scribd, and many more recognized academic repositories.

How to submit the paper?

By Our website

Click Here to Submit Paper Online

By Mail

Send your paper to editor@ijnrd.org

Important Dates for Current issue

Paper Submission Open For: October 2025

Current Issue: Volume 10 | Issue 10 | October 2025

Impact Factor: 8.76

Last Date for Paper Submission: Till 31-Oct-2025

Notification of Review Result: Within 1-2 Days after Submitting paper.

Publication of Paper: Within 01-02 Days after Submititng documents.

Frequency: Monthly (12 issue Annually).

Journal Type: IJNRD is an International Peer-reviewed, Refereed, and Open Access Journal with Transparent Peer Review as per the new UGC CARE 2025 guidelines, offering low-cost multidisciplinary publication with Crossref DOI and global indexing.

Subject Category: Research Area

Call for Paper: More Details