Isolation & Molecular Identification of Staphylococcus equorum 16S rRNA gene from Poultry Feces samples of Latur Region Poultry Farm (Ms) India

Isolation & Molecular Identification of Staphylococcus equorum 16S rRNA gene from Poultry Feces samples of Latur Region Poultry Farm (Ms) India

Registration ID: IJNRD_223700

Published ID: IJNRD2406325

DOI: http://doi.one/10.1729/Journal.40172

Dr. Sudarshan Sambhajirao Pedge

Key Words: Poultry Feces, 16S rRNA gene, BLAST Tool,

ABSTRACT: The accumulation of poultry waste, comprising various materials such as broiler and layer carcasses, feathers, bones, blood, hatchery debris, and deceased birds, poses significant environmental and health concerns. These waste materials can lead to microbial contamination, foul odours, and the proliferation of pests like flies and rodents, thereby contributing to environmental pollution. The Samples were collected according to standard microbiological procedures during study period September 2022 to June 2023. 2-5 gm of samples were collected in sterile screw cap tube using sterile spatula and immediately transported with specially prepared ice box to the laboratory for further analysis. Biochemical assays confirmed the bacterial specimens, and molecular characterization was conducted through polymerase chain reaction (PCR) and sequencing of the 16S rRNA gene of S. equorum. The newly sequenced 16S rRNA gene sequences demonstrated 100% homology to S. equorum, as analyzed using the NCBI-BLAST tool. Phylogenetic analysis and nucleotide base composition studies were performed using 60 sequences of the 16S rRNA gene from various Staphylococcus isolates, including Staphylococcus equorum. For this purpose, 16S rRNA gene sequences were retrieved from NCBI in FASTA format. The phylogenetic analysis, conducted using the Maximum Likelihood method, revealed the relationships and percent similarity of the Staphylococcus equorum 16S rRNA gene.

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"Isolation & Molecular Identification of Staphylococcus equorum 16S rRNA gene from Poultry Feces samples of Latur Region Poultry Farm (Ms) India", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.9, Issue 6, page no.d313-d322, June-2024, Available :https://ijnrd.org/papers/IJNRD2406325.pdf

Volume 9 Issue 6, June-2024

Pages : d313-d322

Paper Reg. ID: IJNRD_223700

Published Paper Id: IJNRD2406325

Downloads: 000121170

Research Area: Life Sciences 

Country: Latur, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406325.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406325

DOI: http://doi.one/10.1729/Journal.40172

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

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