COMPARATIVE STUDY OF DNA YIELD FROM VARIOUS BLOOD SAMPLES COLLECTED UNDER DIFFERENT CONDITIONS

COMPARATIVE STUDY OF DNA YIELD FROM VARIOUS BLOOD SAMPLES COLLECTED UNDER DIFFERENT CONDITIONS

Registration ID: IJNRD_223182

Published ID: IJNRD2406216

DOI: Click Here to Get

Saraswath Ruchita Sharma , Vinaya Bodavula , Dr H R Bhargava

Keywords; DNA Extraction, DNA analysis, Quantitative estimation, Agarose gel electrophoresis, Quantification, Purification.

Forensic/DNA analysis is completely based on the extraction of DNA from the biological samples available at crime scenes. Blood being one of the commonly available sample sources from crime scenes, is generally used for DNA extractions. DNA profiling and sequencing techniques depend on the quantity of DNA below which the analysis becomes practically not possible. Thus, extraction methods used for sample processing must be accurate and specific based on the type of sample obtained to yield maximum DNA from available samples. Current work involves analysis of different types of blood samples that can be possibly obtained from crime scenes which include 1) blood-stained cloth, 2) blood dried on a la surface, 3) blood absorbed on cotton fiber, 4) blood spread on a floor and 5) fresh blood obtained from suspect. All the samples were subjected to DNA extraction using different protocols for testing the yield. DNA extracted was subjected to purification, agarose gel run, and quantification. Based on the results obtained it would be possible to suggest a specific method for a given type of sample for maximum extraction yield of DNA.

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"COMPARATIVE STUDY OF DNA YIELD FROM VARIOUS BLOOD SAMPLES COLLECTED UNDER DIFFERENT CONDITIONS", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.9, Issue 6, page no.c184-c196, June-2024, Available :https://ijnrd.org/papers/IJNRD2406216.pdf

Volume 9 Issue 6, June-2024

Pages : c184-c196

Paper Reg. ID: IJNRD_223182

Published Paper Id: IJNRD2406216

Downloads: 000121206

Research Area: Science & Technology

Country: Hyderabad, Telangana, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406216.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406216

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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