Paper Title
COMPARATIVE STUDY OF DNA YIELD FROM VARIOUS BLOOD SAMPLES COLLECTED UNDER DIFFERENT CONDITIONS
Article Identifiers
Authors
Saraswath Ruchita Sharma , Vinaya Bodavula , Dr H R Bhargava
Keywords
Keywords; DNA Extraction, DNA analysis, Quantitative estimation, Agarose gel electrophoresis, Quantification, Purification.
Abstract
Forensic/DNA analysis is completely based on the extraction of DNA from the biological samples available at crime scenes. Blood being one of the commonly available sample sources from crime scenes, is generally used for DNA extractions. DNA profiling and sequencing techniques depend on the quantity of DNA below which the analysis becomes practically not possible. Thus, extraction methods used for sample processing must be accurate and specific based on the type of sample obtained to yield maximum DNA from available samples. Current work involves analysis of different types of blood samples that can be possibly obtained from crime scenes which include 1) blood-stained cloth, 2) blood dried on a la surface, 3) blood absorbed on cotton fiber, 4) blood spread on a floor and 5) fresh blood obtained from suspect. All the samples were subjected to DNA extraction using different protocols for testing the yield. DNA extracted was subjected to purification, agarose gel run, and quantification. Based on the results obtained it would be possible to suggest a specific method for a given type of sample for maximum extraction yield of DNA.
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How To Cite (APA)
Saraswath Ruchita Sharma, Vinaya Bodavula , & Dr H R Bhargava (June-2024). COMPARATIVE STUDY OF DNA YIELD FROM VARIOUS BLOOD SAMPLES COLLECTED UNDER DIFFERENT CONDITIONS. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), c184-c196. https://ijnrd.org/papers/IJNRD2406216.pdf
Issue
Volume 9 Issue 6, June-2024
Pages : c184-c196
Other Publication Details
Paper Reg. ID: IJNRD_223182
Published Paper Id: IJNRD2406216
Downloads: 000121985
Research Area: Science & Technology
Country: Hyderabad, Telangana, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2406216.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406216
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition
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