Qualitative Assessment Of Processed Honey In Comparison With Formalin As An Alternative Fixative

Registration ID: IJNRD_223139

Published ID: IJNRD2406071

DOI: http://doi.one/10.1729/Journal.39825

RUTTALA SAI PRAVEENA , P. CHANDRA SEKHAR , K. KIRAN KUMAR , K. VATSALYA , L. SANJANA

Formalin, Processed honey, Cellular outline, Cytoplasmic preservation, Blood capillary preservation.

Fixation is an initial and important step in tissue processing which preserves the tissues in a life-like state, prevents bacterial putrefaction, prevents autolysis, and increases the refractive index of the tissue. This study has been done to evaluate the fixation ability of honey in oral tissues and to compare the fixative efficacy of honey and formalin. The study group comprised of 21 tissue samples. Each tissue was cut into 2 segments and one was immediately fixed in 10% Formalin and the other segment in Processed Honey (in the ratio of 70:30- honey and distilled water), for 24 hours at room temperature. The sections were assessed for cytoplasmic, nuclear details & staining quality under light microscope and evaluated accordingly by two observers. Results were analysed by Chi-square test. Inter-observer variability was determined by Kappa statistics. When all the stained sections were assessed for the parameters, there was no statistically significance difference between tissues fixed in processed honey compared to formalin (P> 0.05). Kappa score is 0.755, it falls within the "substantial agreement" range, indicating that the observers have a high level of agreement. Our results suggest that processed honey can be used as a safe alternative for formalin. It can be successfully adopted in routine histopathology laboratories in place of formalin. Hence honey can be considered as an equally effective formalin substitute.

Published Paper   E-Certificate

RUTTALA SAI PRAVEENA, P. CHANDRA SEKHAR, K. KIRAN KUMAR, K. VATSALYA, & L. SANJANA (June-2024). Qualitative Assessment Of Processed Honey In Comparison With Formalin As An Alternative Fixative. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), a728-a734. http://doi.one/10.1729/Journal.39825

Volume 9 Issue 6, June-2024

Pages : a728-a734

Paper Reg. ID: IJNRD_223139

Published Paper Id: IJNRD2406071

Downloads: 000122002

Research Area: Medical Science

Country: GUNTUR, ANDHRA PRADESH, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406071.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2406071

Crossref DOI: http://doi.one/10.1729/Journal.39825

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave | IJNRD.ORG | IJNRD.COM | IJPUB.ORG

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

Call For Paper - Volume 10 | Issue 10 | October 2025

IJNRD is a Scholarly Open Access, Peer-reviewed, and Refereed Journal with a High Impact Factor of 8.76 (calculated by Google Scholar & Semantic Scholar | AI-Powered Research Tool). It is a Multidisciplinary, Monthly, Low-Cost Journal that follows UGC CARE 2025 Peer-Reviewed Journal Policy norms, Scopus journal standards, and Transparent Peer Review practices to ensure quality and credibility. IJNRD provides indexing in all major databases & metadata repositories, a citation generator, and Digital Object Identifier (DOI) for every published article with full open-access visibility.

The INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to advance applied, theoretical, and experimental research across diverse fields. Its goal is to promote global scientific information exchange among researchers, developers, engineers, academicians, and practitioners. IJNRD serves as a platform where educators and professionals can share research evidence, models of best practice, and innovative ideas, contributing to academic growth and industry relevance.

Indexing Coverage includes Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar (AI-Powered Research Tool), Microsoft Academic, Academia.edu, arXiv.org, ResearchGate, CiteSeerX, ResearcherID (Thomson Reuters), Mendeley, DocStoc, ISSUU, Scribd, and many more recognized academic repositories.

How to submit the paper?

Important Dates for Current issue

Paper Submission Open For: October 2025

Current Issue: Volume 10 | Issue 10 | October 2025

Impact Factor: 8.76

Last Date for Paper Submission: Till 31-Oct-2025

Notification of Review Result: Within 1-2 Days after Submitting paper.

Publication of Paper: Within 01-02 Days after Submititng documents.

Frequency: Monthly (12 issue Annually).

Journal Type: IJNRD is an International Peer-reviewed, Refereed, and Open Access Journal with Transparent Peer Review as per the new UGC CARE 2025 guidelines, offering low-cost multidisciplinary publication with Crossref DOI and global indexing.

Subject Category: Research Area

Call for Paper: More Details