Pharmacognostic Evaluation, and GC-MS Profiling of Landolphia dulcis, Sabine ex G.Don (Apocynaceae) Leaf Methanol

Registration ID: IJNRD_221441

Published ID: IJNRD2406001

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Chikeokwu Ikenna , Michael Onyegbulam Chukwuma , Theodora Chidinma MBA

Landolphia dulcis, GC-MS, pharmacognostic evaluation, Standardization

Introduction: Standardization is the investigation and documentation of unique and peculiar characters and properties which identify and separate a plant from others even members of the same family. Landolphia dulcis, Sabine ex G.Don belongs to the family Apocynaceae which is known to contain many secondary metabolites and have many pharmacological activities. Standardization refers to the body of information and control necessary to product material of reasonable consistency. Methods of standardization take into consideration all aspects that contribute to the quality of the herbal drugs, namely correct identity of the sample, organoleptic evaluation, pharmacognostic evaluation, Physicochemical quantitative evaluation (LOD, ash values, extractive values), phytochemical evaluation (e.g. GC-MS), microscopy. Of these, the phytochemical profile is of special significance since it has a direct bearing on the activity of the herbal drugs. This research aimed to provide information on standardization parameters and GC-MS profile of the leaves of Landolphia dulcis, Sabine ex G.Don. Methodology; The fresh leaf was used for quantitative microscopy studies while a part of the collection was dried under shade, powdered and used for powder microscopy, physico-chemical analysis. Standard pharmacopoeia methods were followed in evaluation of pharmacgnstic parameters. GC-MS, profiling was carried out using standard methods and equipment. Results: Physicochemical properties were within normal pharmacopeia limits. Total ash was 7.40 ± 0.02 %, methanol had extractive value of 9.27 ± 0.05 % while water gave an extractive value of 8.91 ± 0.04. Leaf microscopy revealed the presence of prism-shape Calcium oxalates crystals, Stomata index (%) 18.75 ± 0.38, Stomata number 15.25 ± 0.67, and palisade ratio 12.50 ± 0.65. The leaf has paracytic stomata type which was only present on the dorsal (adaxial) surface. GC-MS result showed the presence of 45 compounds with diverse activities. Conclusion: These study have provided basic and fundamental information on the plant, and justification for its safe use in ethno medicine.

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Chikeokwu Ikenna, Michael Onyegbulam Chukwuma , & Theodora Chidinma MBA (June-2024). Pharmacognostic Evaluation, and GC-MS Profiling of Landolphia dulcis, Sabine ex G.Don (Apocynaceae) Leaf Methanol. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(6), a1-a16. https://ijnrd.org/papers/IJNRD2406001.pdf

Volume 9 Issue 6, June-2024

Pages : a1-a16

Paper Reg. ID: IJNRD_221441

Published Paper Id: IJNRD2406001

Downloads: 000121990

Research Area: Pharmacy

Country: Nsukka, Enugu, Nigeria

Published Paper PDF: https://ijnrd.org/papers/IJNRD2406001.pdf

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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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