Paper Title
Chilli Anther culture for haploid and doubled haploid production
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Registration ID: IJNRD_222493
Published ID: IJNRD2405811
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Keywords
Introduction, Material and method, Description of Experimental Area Results, Discussion, Conclusion
Abstract
Plants with a gametophytic chromosome number are called haploids, while those with two chromosomes duplicated are called doubled haploids. When compared to the conventional breeding method that uses several generations of egging, the production of haploids and doll bled haploids (DHs) through gametic embryo gene allows for the single-step development of complete homozygous lines from heterozygous parent plants, significantly reducing the time required to produce homozygous plants. Agriculture systems have been greatly impacted by the development of haploidy technology and protocols to generate homozygous plants, as well as the generation of haploids and DHs, which is a particularly attractive biotechnological tool. These biotechnologies now play a crucial role in the breeding programs of numerous crops that are significant to agronomy. Local chilli cultivars in Vietnam possess a unique scent and pungency. There haven't been many reports of pure line development from pollen culture in the local hot chilli, though. The purpose of this study was to determine how flower bud size related to the developmental phases of microspores and how changes in culture media concentration affected the impact of plant growth regulators on in vitro androgenesis. Based on the size of the petals and sepals, flower buds were gathered at random and visually sorted into three stages. The anthers were subsequently cultivated on MS basal medium with varying concentrations of BA (0.5 - 1.5 mg/L), kinetin (1.0 - 3.0 mg/L), and NAA (0.1 - 0.7 mg/L). The findings demonstrated that the 2.5 mm long, light violet anthers were made up of 80% haploid and 20% Dihaploid anthers. With in vitro anther culture, twelve different pepper genotypes (Capsicum annuum L.) were studied to ascertain the impact of genotype and medium on haploid plant production. After being cultivated in O1 and O2, the buds' cultured conditions were found to have an impact on the commencement of plantlets, embryonic development, and anther development. The cv. Flinta F1 genotype produced the greatest rate of 12.79% when the anther developmental status was assessed, whereas the SU-31 genotype produced the lowest result, 2.47%. With an astounding 25.0% growth rate in the O2 medium, the cv. Dolphin variety yielded the highest growth rates. Out of the eight embryos and five plants, the cultivar Dolphin had the best genotype.
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Janhavi manoj magar & Dr sarosha khan (May-2024). Chilli Anther culture for haploid and doubled haploid production. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(5), I90-I101. https://ijnrd.org/papers/IJNRD2405811.pdf
Issue
Volume 9 Issue 5, May-2024
Pages : I90-I101
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Paper Reg. ID: IJNRD_222493
Published Paper Id: IJNRD2405811
Research Area: Science & Technology
Author Type: Indian Author
Country: hinganghat, maharashtra, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2405811.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2405811
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