Paper Title
Microbial assessment of Offices that Uses fan and Air conditioners in Rhema University, Nigeria, Aba.
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Registration ID: IJNRD_221222
Published ID: IJNRD2405427
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Keywords
Air Conditioners, Fans, Fungi, Bacteria, antibiotics susceptibility.
Abstract
The aim of this study was to determine the microbes present in the indoor air of offices that use fan and offices that use Air conditions (AC) in Rhema University, Aba. A total of 20 offices were used to carry out this study. 10 of these offices used only fan while the other 10 used only Air conditioners. Plates of nutrient agar, MacConkey agar and Czapex dox agar each, freshly prepared, where exposed in these offices at nose level for 20minutes. They were then aseptically wrapped and transported back to the laboratory immediately for incubation. MacConkey and Nutrient agar plates were incubated at 37oC for 24hours while Czapex dox agar was incubated at 280C for 7-21days. Mean bacterial counts obtained from the AC offices had a total hetertrophic count of 18-177cfu/m3 and total coliform bacteria count in 6 offices had a range of 6-32 cfu/ml3 while total fungi count recorded in 8 offices had a range of 12-46 cfu/ml3. In the offices that uses fan, total heterotrophic bacteria count had a range of 26-144 cfu/m3 in all the offices and a total coliform bacteria count in 5 offices had a range of 8-92cfu/m3 while total fungi count was recorded in 8 offices having a range of 2-46cfu/m2. The gram positive bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus species, Micrococcus Species and Bacillus species while the gram negative bacteria isolated were Acinetobacter, Klebsiella pneumoniae, and Escherichia coli. The fungi isolated were Aspergillus niger, Penicillium species, Rhizopus, Candida species, Trycophyton, and Cladosporium species. Staphylococcus aureus (100%) was the bacteria with the highest occurrence while Aspergillus niger (100%) was the fungi with the highest occurrence. Potential pathogenicity testing of the bacteria isolates showed that out of 8 bacteria that was isolated, 7 (87.5%) were beta hemolytic. Antibiotic susceptibility showed that the bacteria were sensitive to the drugs used which were Tarvid, Reflacine, Rifampicin Ciprofloxacin, Augumentin, Chloramphenicol, Ampiclox Gentamycin, Levofloxacin, Amoxil, Streptomycin, Ceporex, Nalidixix Acid, Septrin and Ampicilin This study has shown the presence of fungi and bacteria of public health importance in offices that uses fan and Air conditioners, therefore an intense cleaning plan should be put in place to reduce their proliferation.
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How To Cite (APA)
Ekeleme Jessica Ezinne, Okoro Immaculata, Ude, Ngozi Obioma, Izuchukwu, Ofoegbu, Nnamdi Jude, & Okoronkwo, Paul Adimchino (May-2024). Microbial assessment of Offices that Uses fan and Air conditioners in Rhema University, Nigeria, Aba.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(5), e228-e239. https://ijnrd.org/papers/IJNRD2405427.pdf
Issue
Volume 9 Issue 5, May-2024
Pages : e228-e239
Other Publication Details
Paper Reg. ID: IJNRD_221222
Published Paper Id: IJNRD2405427
Research Area: Biological Science
Author Type: Foreign Author
Country: Aba, Abia, Nigeria
Published Paper PDF: https://ijnrd.org/papers/IJNRD2405427.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2405427
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