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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | DEVELOPMENT OF AN EFFICIENT PROTOCOL FOR IN VITRO REGENERATION OF CAPSICUM (CAPSICUM ANNUUM L.) FROM NODAL EXPLANTS |
| Authors Name: | Manu Vivek , Manisha Thakur |
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IJNRD_213898
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| Published Paper Id: | IJNRD2402218 |
| Published In: | Volume 9 Issue 2, February-2024 |
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| Abstract: | The successful implementation of any plant biotechnological approach relies heavily on the establishment of a robust plant regeneration system within the target crop. In this study, the focus was on achieving in vitro regeneration utilizing nodal explants obtained from aseptically raised seedlings of Capsicum namely Solan Bharpur. To optimize germination, seeds underwent various treatments and were subsequently decontaminated before being cultured in vitro on half-strength basal MS medium. Notably, seeds soaked in distilled water supplemented with 1 mg/l GA3 for one day prior to sowing exhibited the most significant impact, resulting in maximum germination rates within a relatively short period. Tissue culture responses were found to be influenced by specific combinations of growth regulators utilized. MS medium supplemented with 2mg/L BAP exhibited shoot regeneration within three weeks. The highest number of shoots per explant (4.0), and average shoot length of 4.5cm was observed in MS medium supplemented with 0.5 mg/l BAP along with 1.0 mg/l Kinetin. Subsequent culturing of regenerated shoots on MS media supplemented with 1 mg/l IBA and 1.5mg/L IAA led to 86% rooting. During the hardening phase, where plantlets were transferred to pots containing sterile coco-peat , the survival percentage was notably higher (89.6%). |
| Keywords: | Capsicum, Tissue culture, Micropropagation, Regeneration, Bell pepper |
| Cite Article: | "DEVELOPMENT OF AN EFFICIENT PROTOCOL FOR IN VITRO REGENERATION OF CAPSICUM (CAPSICUM ANNUUM L.) FROM NODAL EXPLANTS", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.9, Issue 2, page no.c166-c172, February-2024, Available :http://www.ijnrd.org/papers/IJNRD2402218.pdf |
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| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRD2402218 Registration ID: 213898 Published In: Volume 9 Issue 2, February-2024 DOI (Digital Object Identifier): Page No: c166-c172 Country: Neri, Hamirpur, HP, HIMACHAL PRADESH, India Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2402218 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2402218 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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