PHARMACOGNOSTICAL EVALUATION OF ALLIUM CEPA USING UV-VISIBLE SPECTROSCOPY, HPTLC AND POWDER MICROSCOPIC METHODS

Registration ID: IJNRD_213175

Published ID: IJNRD2401314

DOI: Click Here to Get

Jayalekshmi K S , Dr.M.S. Latha

Allium cepa, pharmacognostic study, microscopic features, physicochemical parameters, UV-Visible spectra, HPTLC profile.

Allium cepa commonly known as Onion is consumed as food across the world and also used to treat inflammatory diseases in traditional systems of medicine. The present study provides a detailed pharmacognostic evaluation based on its physicochemical, macroscopic, microscopic and chromatographic properties. The physicochemical parameters such as loss on drying, solubility in different solvents, ash content, acid insoluble ash, water soluble ash, volatile oil, fibre content etc. were determined by standard methods. Anatomical features of the stem of Allium cepa were determined. For this the sample was fixed in FAA, cast into paraffin blocks and sectioned with the help of Rotary Microtome. The stomata morphology, venation pattern and trichome distribution were studied. Microscopic descriptions of tissues were supplemented with micrographs wherever necessary. Photographs of different magnifications were taken with Nikon Labphot 2 Microscopic Unit. Powder microscopy was carried out using standard methods. HPTLC profile of the ethyl acetate plant extract was carried out in short UV, long UV and using vanillin-sulphuric acid as detection reagent. The Rf values of the spots developed were noted which is an important parameter for identification of plant materials. The pharmacognostical parameters along with the HPTLC profile may be utilized to identify the drug material and for laying down the pharmacopoeial standards.

Published Paper   E-Certificate

Jayalekshmi K S & Dr.M.S. Latha (January-2024). PHARMACOGNOSTICAL EVALUATION OF ALLIUM CEPA USING UV-VISIBLE SPECTROSCOPY, HPTLC AND POWDER MICROSCOPIC METHODS. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(1), d111-d119. https://ijnrd.org/papers/IJNRD2401314.pdf

Volume 9 Issue 1, January-2024

Pages : d111-d119

Paper Reg. ID: IJNRD_213175

Published Paper Id: IJNRD2401314

Downloads: 000121984

Research Area: Chemistry

Country: Alappuzha, Kerala, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2401314.pdf

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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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