TYPE 2 DIABETES: EVALUATING THE EFFECTS OF CLARAMINE ON INSULIN- MEDIATED GLUCOSE REGULATION IN HEPATOMA (HEPG2) CELL LINE.

Registration ID: IJNRD_205279

Published ID: IJNRD2311078

DOI: Click Here to Get

DR. LAMIDI, TAJUDEEN BABATUNDE , KILEDU, ISREAL AHMADU

Keywords: Diabetes, Insulin, PTPs, Claramine, Hepatoma cell line, Metabolic disease

ABSTRACT Diabetes is a chronic, metabolic disease characterized by elevated levels of blood glucose (or blood sugar), which leads over time to serious damage to the heart, blood vessels, eyes, kidneys and nerves. The most common is type 2 diabetes, usually in adults, which occurs when the body becomes resistant to insulin or doesn't make enough insulin. Protein tyrosine phosphatises (PTPs) are critical antagonist of insulin receptor (IR) signalling. Their inhibition with pharmacological inhibitory agents can play the role of insulin thus facilitating the uptake of glucose and the inhibition of lipolysis. Elevated PTPIB activity underlies insulin resistance in type 2 diabetes. Several experiments have shown that PTPIB is implicated in glucose regulation obesity and type-2 diabetes. Claramine is a novel synthetic chemical agent which possess selective inhibitory activity against PTPIB. This research was aimed at unveiling the effects of claramine on insulin-mediated glucose regulation in Hepatoma (HepG2) cell lines. Research materials including Hep G2 cell line, T-75 flasks, Eppendorf tubes, 96-well plates, Bradford reagent test kit, cell culture media (Dulbecco's Modified Eagle Medium) (DMEM) and all reagents for buffer preparation was purchased. First experiment was conducted to test the viability of the HepG 2 cell lines using standard procedure. Second round of experiment was conducted to culture the HepG 2 cells at 37OC. The cells were divided into 6 wells plates and allowed to adhere for 24 hours. After adherence, the cells were treated with high glucose and different concentrations of insulin and claramine. The determination of glucose utilisation by the cells was done with multiscan-FC spectrophotometer at 540nm and the amount of glucose utilised by the cell was calculated and use for protein normalisation. Combination of 50nm of insulin with various concentrations of claramine showed trend in the uptake of glucose by Hep2 cells with highest uptake at 2.0um of claramine. However, with 100nm insulin there was relative little and irregular variation in glucose uptake by HepG 2 cells with different concentrations of claramine. Also, glucose utilisation with respect to joint variation of insulin and claramine concentrations showed increase uptake at 50nm insulin and 1um claramine. Hepatoma cell lines can be used to investigate the glucose uptake and glucose metabolism in hepatocyte. claramine is a first class highly selective inhibitor of protein tyrosine phosphtase IB (PTPIB). It is therefore eminent that inhibitors of PTPIB plays important role as efficient therapeutic target for type-II diabetes and obesity by increasing insulin and leptin sensitivity.

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DR. LAMIDI, TAJUDEEN BABATUNDE & KILEDU, ISREAL AHMADU (November-2023). TYPE 2 DIABETES: EVALUATING THE EFFECTS OF CLARAMINE ON INSULIN- MEDIATED GLUCOSE REGULATION IN HEPATOMA (HEPG2) CELL LINE.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(11), a710-a719. https://ijnrd.org/papers/IJNRD2311078.pdf

Volume 8 Issue 11, November-2023

Pages : a710-a719

Paper Reg. ID: IJNRD_205279

Published Paper Id: IJNRD2311078

Downloads: 000121988

Research Area: Pharmacy

Country: BALI, TARABA , Nigeria

Published Paper PDF: https://ijnrd.org/papers/IJNRD2311078.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2311078

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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