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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | Rapid Detection and Identification of viral pathogens in fish aquaculture by PCR Protocols |
| Authors Name: | Dr. Rekhani jyoti Pal , Gulshan kumar |
| Download E-Certificate: | Download |
| Author Reg. ID: |
IJNRD_203808
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| Published Paper Id: | IJNRD2308246 |
| Published In: | Volume 8 Issue 8, August-2023 |
| DOI: | |
| Abstract: | Fish diseases pose a universal threat to the ornamental fish industry, aquaculture, and public health. They can be caused by many organisms, including bacteria, fungi, viruses, and protozoa. Early detection of the causative agent which resulted in massive mortalities is a crucial aspect in the health management of fish aquaculture. Several diagnostic techniques have been developed for several pathogenic diseases and the use of molecular approaches is widely accepted. The present study optimized PCR protocols to detect a number of commercially important viral pathogens, including Infectious haematopoietic necrosis virus (IHNV), Viral haemorrhagic septicaemia virus (VHSV), Spring viraemia of carp virus (SVCV), Infectious salmon anaemia virus (ISAV), Koi herpes virus (KHV), Epizootic haematopoietic necrosis virus (EHNV), and other ranavirus. Red sea bream iridoviral and other megalocytivirus, viral nervous necrosis and other nodavirus (VNNV) in fishes as well as to ensure that these PCR assays are suited to Indian conditions. Many techniques have been developed, each requiring its own protocol, equipment, and expertise. In this regard, the advent of molecular biology, especially polymerase chain reaction (PCR), provides alternative means for detecting and identifying fish pathogens. The optimized PCR protocols for the detection of various viral pathogens in fish are sensitive as well as site-specific. These assays can be used as a management tool for the prevention and control of viral diseases in fish aquaculture in the country through early detection of the pathogen. In this review, recent advances in molecular fish pathogen diagnosis are discussed with an emphasis on nucleic acid-based detection and identification techniques. Major features and applications of current predominant methods and promising methods likely to impact future fish disease control and prevention are outlined. |
| Keywords: | diseases, diagnostics, pond culture, virus, IHNV, VHSV, SVCV. |
| Cite Article: | "Rapid Detection and Identification of viral pathogens in fish aquaculture by PCR Protocols", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 8, page no.c321-c331, August-2023, Available :http://www.ijnrd.org/papers/IJNRD2308246.pdf |
| Downloads: | 000118751 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRD2308246 Registration ID: 203808 Published In: Volume 8 Issue 8, August-2023 DOI (Digital Object Identifier): Page No: c321-c331 Country: SHAHBAD MARKANDA, HARYANA, India Research Area: Life Sciences Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2308246 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2308246 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
Journal Starting Year (ESTD) : 2016
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