Rapid Detection and Identification of viral pathogens in fish aquaculture by PCR Protocols

Rapid Detection and Identification of viral pathogens in fish aquaculture by PCR Protocols

Registration ID: IJNRD_203808

Published ID: IJNRD2308246

DOI: Click Here to Get

Dr. Rekhani jyoti Pal , Gulshan kumar

diseases, diagnostics, pond culture, virus, IHNV, VHSV, SVCV.

Fish diseases pose a universal threat to the ornamental fish industry, aquaculture, and public health. They can be caused by many organisms, including bacteria, fungi, viruses, and protozoa. Early detection of the causative agent which resulted in massive mortalities is a crucial aspect in the health management of fish aquaculture. Several diagnostic techniques have been developed for several pathogenic diseases and the use of molecular approaches is widely accepted. The present study optimized PCR protocols to detect a number of commercially important viral pathogens, including Infectious haematopoietic necrosis virus (IHNV), Viral haemorrhagic septicaemia virus (VHSV), Spring viraemia of carp virus (SVCV), Infectious salmon anaemia virus (ISAV), Koi herpes virus (KHV), Epizootic haematopoietic necrosis virus (EHNV), and other ranavirus. Red sea bream iridoviral and other megalocytivirus, viral nervous necrosis and other nodavirus (VNNV) in fishes as well as to ensure that these PCR assays are suited to Indian conditions. Many techniques have been developed, each requiring its own protocol, equipment, and expertise. In this regard, the advent of molecular biology, especially polymerase chain reaction (PCR), provides alternative means for detecting and identifying fish pathogens. The optimized PCR protocols for the detection of various viral pathogens in fish are sensitive as well as site-specific. These assays can be used as a management tool for the prevention and control of viral diseases in fish aquaculture in the country through early detection of the pathogen. In this review, recent advances in molecular fish pathogen diagnosis are discussed with an emphasis on nucleic acid-based detection and identification techniques. Major features and applications of current predominant methods and promising methods likely to impact future fish disease control and prevention are outlined.

Published Paper   E-Certificate

"Rapid Detection and Identification of viral pathogens in fish aquaculture by PCR Protocols", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.8, Issue 8, page no.c321-c331, August-2023, Available :https://ijnrd.org/papers/IJNRD2308246.pdf

Volume 8 Issue 8, August-2023

Pages : c321-c331

Paper Reg. ID: IJNRD_203808

Published Paper Id: IJNRD2308246

Downloads: 000121187

Research Area: Life Sciences 

Country: SHAHBAD MARKANDA, HARYANA, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2308246.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2308246

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

Call For Paper - Volume 10 | Issue 8 | August 2025

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