INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
In recent years, there has been an increase in interest in employing plant-based mixes and natural products as anti-microbial agents. The goal of this study was to determine how effective Allium sativum extracts against S. aureus and E. coli were at killing bacteria. The garlic cloves were peeled and ground into a puree. The garlic extracts were homogenised and combined with distilled water to produce four different concentrations of garlic extract: 25%, 50%, 75%, and 100%. S. aureus and E. coli were used to test the Aqueous Garlic Extract's (AGE) antibacterial activity against gram-positive and gram-negative bacterial isolates. Agar Well-Diffusion Method evaluation of the antimicrobial effects of various doses of aqueous garlic extract (AGE). In order to combat S. aureus and E. coli, garlic extract was employed in concentrations ranging from 100% to 5% (1ml/mL, 0.5 ml/mL, 0.25 ml/mL, and 0.125 ml/mL). S. aureus and E. coli were evaluated for resistance to the extract. Compared to both microorganisms, various anti-microbial activity were seen in all concentrations of garlic extract. However, in cases of both S. aureus and E. coli, the area of suppression produced by 100% of garlic extract was greater compared to those with lesser garlic strength. The findings of this study imply that garlic extract has potent antimicrobial elements. Investigation is necessary to enhance extraction techniques and identify the anti-microbial effects of garlic extract on various bacterial species. Further research revealed that the anti-microbial efficacy of aqueous garlic extract (AGE) depends on temperature and duration. According to the findings of this study, garlic is effective against pathogenic bacteria and has anti-microbial action against S. aureus and E. coli.
Keywords:
Allium sativum , Anti-microbial activity, Agar well-diffusion method, AGE,
Cite Article:
"A research : Determination of Anti-microbial Activity ", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 6, page no.b563-b579, June-2023, Available :http://www.ijnrd.org/papers/IJNRD2306164.pdf
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ISSN:
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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