Isolation and screening of bacteria from Jeevamrutha and Ghanajeevamrutha against the pathogen Sclerotium rolfsii for their biocontrol activity and their biochemical characterization

Registration ID: IJNRD_194610

Published ID: IJNRD2306087

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SAHANA S.B. , SREENIVASA M.N. , C.R. PATIL , Gurudatt M. Hegde

Keywords: Jeevamrutha, Ghanajeevamrutha, Sclerotium rolfsii, per cent inhibition, biochemical characterization.

Abstract: An experiment was conducted to study the biocontrol activity of bacterial isolates from Jeevamrutha and Ghanajeevamrutha at the University of Agricultural Sciences, Dharwad. Jeevamrutha and Ghanajeevamrutha are cheaper and eco-friendly organic preparations made of desi cow products. These are efficient plant growth stimulants that enhance the biological efficiency of crops. Soil borne plant pathogens cause serious losses to crop plants reducing the yield to the extent of 75-80 per cent. In this context, bacteria were isolated from the Jeevamrutha and Ghanajeevamrutha (prepared at laboratory scale) and were subjected for dual culture plate assay on Potato Dextrose Agar medium against the plant pathogen Sclerotium rolfsii. Zone of inhibition was calculated by comparing to the pathogen growth on the control plate. The isolated bacteria showed different degrees of antagonism (28.75-47.78 per cent) against the pathogen, among them the isolates J1 (isolate from Jeevamrutha) and GJ1(isolated from Ghanajeevamrutha) showed significantly the highest inhibition percentage of 47.78 per cent and 36.55 per cent respectively against the pathogen Sclerotium rolfsii. Further the isolates were subjected for morphological and biochemical characterization, the bacterial isolates were found to be a mixture of both Gram positive and Gram-negative bacteria with varying shapes. Most of them were positive for citrate utilization test indicating citrate as the sole source of carbon used by the isolates, only few were positive for PAL test i.e., Phenylalanine ammonia lyase test.

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SAHANA S.B., SREENIVASA M.N., C.R. PATIL, & Gurudatt M. Hegde (June-2023). Isolation and screening of bacteria from Jeevamrutha and Ghanajeevamrutha against the pathogen Sclerotium rolfsii for their biocontrol activity and their biochemical characterization. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(6), a754-a764. https://ijnrd.org/papers/IJNRD2306087.pdf

Volume 8 Issue 6, June-2023

Pages : a754-a764

Paper Reg. ID: IJNRD_194610

Published Paper Id: IJNRD2306087

Downloads: 000121994

Research Area: Life Sciences 

Country: dharwad , karnataka, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2306087.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2306087

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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