Antibacterial activity of Sand crab (Emerita asiatica) hemolymph against Asian seabass (Lates calcarifer) infected with Aeromonas salmonicida

Registration ID: IJNRD_196913

Published ID: IJNRD2305689

DOI: http://doi.one/10.1729/Journal.34434

F.S. Fazil Ahamed , Kundan Basu , Ragunathan M.G , Jayanthi.J

Antibacterial activity, hemolymph, Emerita asiatica, Aeromonas salmonicida, Lates calcarifer, histopathology.

ABSTRACT In the current study, crude hemolymph of sand crab (Emerita asiatica) was used to immunize Asian seabass larvae against Aeromonas salmonicida infection. The bacteria isolated from the infected fish was provisionally identified as A. salmonicida using its biological, biochemical, physiological and structural characteristics. The study of the 16S rRNA gene using PCR provided additional confirmation. Hemolymph was extracted from the hemocoel of the E. asiatica crab and was tested for its anti-bacterial function using the well diffusion method. A group of fishes (Asian seabass) were treated with the hemolymph of E. asiatica and then challenged with the bacteria after the fourth day of booster. The Hemolymph exhibited inhibition zones ranging from 0.6 cm to 3.33 cm diameter. It was noted that the Relative Percentage Survival (RPS) was 80% against the bacteria. To examine the structural alterations in various organs, including the kidney, liver, gill, and muscles, histopathological examination was done on healthy control, experimentally infected fish, and treated larvae. The infected gill showed clubbing and lamellar fusion. The infected kidney exhibited leucocyte infiltration, tubular breakdown, and hemorrhage. Infected liver cells exhibited severe hepatic sinusoidal congestion, necrosis, and internal hemorrhage. Multiple muscle filaments were broken up in the muscle cells. The fish that had been treated showed significantly less symptoms and suffered less cellular organ damage, confirming the hemolymph's antibacterial properties. As a result, the current study proved that E. Asiatica's haemolymph is a potential source of powerful antibacterial compounds and can be used as an easily accessible natural immunostimulator to fight against bacterial infections.

Published Paper   E-Certificate

F.S. Fazil Ahamed, Kundan Basu, Ragunathan M.G, & Jayanthi.J (May-2023). Antibacterial activity of Sand crab (Emerita asiatica) hemolymph against Asian seabass (Lates calcarifer) infected with Aeromonas salmonicida. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(5), g724-g731. http://doi.one/10.1729/Journal.34434

Volume 8 Issue 5, May-2023

Pages : g724-g731

Paper Reg. ID: IJNRD_196913

Published Paper Id: IJNRD2305689

Downloads: 000121987

Research Area: Life Sciences 

Country: Chennai, Tamil Nadu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2305689.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2305689

Crossref DOI: http://doi.one/10.1729/Journal.34434

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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