Isolation, Identification and Characterization of Bacteria of Coal Mine Soil at Pandwa of Rajhara Coalfield, Jharkhand

Registration ID: IJNRD_196110

Published ID: IJNRD2305572

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Manoj Kumar Singh , Dr. K.K Mishra

Bacillus cereus, Bhargavaea, cecembensis, Rajhara Coalfield, 16S rRNA, Analysis, soil bacteria

Mining is the process of extracting useful materials from the earth. The dust and noxious fumes inhaled by miners make them vulnerable to pulmonary diseases. The water sources in the region get contaminated due to mining. Dumping of waste and slurry leads to degradation of land, soil, and increase in stream and river pollution. Mining means any naturally occurring substance from earth for useful purposes. During this mining process, several changes take place in the physical, chemical, and microbiological characteristics of soil as a result of storage. Soil is a system, in which constant interface between soil minerals and microorganisms control the physicochemical and biological properties of global ecosystem. Microbial properties have been reported to be useful indicators of soil quality and could possibly serve as assessment criteria of successful rehabilitation of ecologically disturbed areas. The present study was conducted to determine dominant bacterial species in the soils of Sonepur Bazari at Raniganj coalfield. During this study, the soil samples were collected in different sites and the bacteria was isolated, identified biochemically and characterized by phylogenetic analysis using 16SrRNA sequencing technique. Two bacterial isolates, i.e. S-1 and S-2 were obtained by using Nutrient agar medium. The isolates were identified morphologically, biochemically and also by phylogenetic analysis using 16SrRNA sequencing technique. The molecular identification of 16SrRNA gene sequences showed that the isolates had 95% similarity to the genus. The results showed that between the two different strains Bacillus cereus is a common soil bacteria whereas Bhargavaea cecembensis is a newly found bacteria in soil.

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Manoj Kumar Singh & Dr. K.K Mishra (May-2023). Isolation, Identification and Characterization of Bacteria of Coal Mine Soil at Pandwa of Rajhara Coalfield, Jharkhand. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(5), f470-f476. https://ijnrd.org/papers/IJNRD2305572.pdf

Volume 8 Issue 5, May-2023

Pages : f470-f476

Paper Reg. ID: IJNRD_196110

Published Paper Id: IJNRD2305572

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Research Area: Engineering

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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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