Paper Title
Development and Validation of LC-MS/MS method for simultaneous estimation of Metronidazole and Rifaximin in Pharmaceutical dosage form and Characterize degradants
Article Identifiers
Authors
Jignesh Chauhan , Mr.Nachiketa Pandya , Dr. Chaitanya Bhatt
Keywords
Metronidazole, Rifaximin, Stability indicating LC-MS/MS Method, Validation.
Abstract
A simple, rapid, precise and accurate Stability indicating LC-MS/MS method for simulta-neous estimation of Metronidazole and Rifaximin in their combined dosage form has been developed. The separation was achieved by Agilent, Zorbax, C18, (150mm x 4.6mm), 5µm column and Buffer (Ammonium formate, pH4.0): Acetonitrile (75:25) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out. Retention time of Rifaximin and Metroni-dazole were found to be 4.9 min and 2.6 min respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Metronidazole 2-6μg/ml and for Rifaximin 1-3 μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Metronidazole and Rifaximin their Combined Dosage Form. The drug was subjected to stress condition of hydrolysis, oxidation, photolysis and Thermal degradation, Considerable Degradation was found in Alkali and Oxidation degradation. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial combined dosage form.
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How To Cite (APA)
Jignesh Chauhan, Mr.Nachiketa Pandya, & Dr. Chaitanya Bhatt (May-2023). Development and Validation of LC-MS/MS method for simultaneous estimation of Metronidazole and Rifaximin in Pharmaceutical dosage form and Characterize degradants. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(5), c67-c83. https://ijnrd.org/papers/IJNRD2305211.pdf
Issue
Volume 8 Issue 5, May-2023
Pages : c67-c83
Other Publication Details
Paper Reg. ID: IJNRD_194575
Published Paper Id: IJNRD2305211
Downloads: 000121988
Research Area: Pharmacy
Country: Ahmedabad, Gujarat, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2305211.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2305211
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
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