Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma

Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma

Registration ID: IJNRD_185889

Published ID: IJNRD2301098

DOI: http://doi.one/10.1729/Journal.32654

R.N.Sathyaprakash , S.Ravichandran

UV, RP-HPLC, pregablin, duloxetine HCl, method development, validation.

This work is concerned with a new, simple, rapid, and precise UV and RP-HPLC method for determination of pregabalin and duloxetine hydrochloride in both bulk and in capsule. UV determination was carried out using Acetonitrile: water as solvent system. The maximum absorbance was observed at 267nm and 218nm for pregabalin and duloxetine hydrochloride respectively. Chromatographic separation was achieved isocratically using C18 column (250×4.6 mm, 5µm), mobile phase containing Acetonitrile and phosphate buffer (pH 6.8) in the ratio 40:60 maintained at flow rate 1 ml/min. Detection wavelength at 240nm. Retention time of pregabalin was found to be 1.8min and for duloxetine HCl 3.4 min. This method is then used for analysis of formulation. The developed method was validated in terms of ICH guidelines Q2(R1). The validation of proposed method was verified by recovery studies and was found to be satisfactory.

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"Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.8, Issue 1, page no.a871-a882, January-2023, Available :https://ijnrd.org/papers/IJNRD2301098.pdf

Volume 8 Issue 1, January-2023

Pages : a871-a882

Paper Reg. ID: IJNRD_185889

Published Paper Id: IJNRD2301098

Downloads: 000121254

Research Area: Pharmacy

Country: Chengalpattu, Tamilnadu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2301098.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2301098

DOI: http://doi.one/10.1729/Journal.32654

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to explore advances in research pertaining to applied, theoretical and experimental Technological studies. The goal is to promote scientific information interchange between researchers, developers, engineers, students, and practitioners working in and around the world. IJNRD will provide an opportunity for practitioners and educators of engineering field to exchange research evidence, models of best practice and innovative ideas.

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