INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
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Paper Title:
Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma
This work is concerned with a new, simple, rapid, and precise UV and RP-HPLC method for determination of pregabalin and duloxetine hydrochloride in both bulk and in capsule. UV determination was carried out using Acetonitrile: water as solvent system. The maximum absorbance was observed at 267nm and 218nm for pregabalin and duloxetine hydrochloride respectively. Chromatographic separation was achieved isocratically using C18 column (250×4.6 mm, 5µm), mobile phase containing Acetonitrile and phosphate buffer (pH 6.8) in the ratio 40:60 maintained at flow rate 1 ml/min. Detection wavelength at 240nm. Retention time of pregabalin was found to be 1.8min and for duloxetine HCl 3.4 min. This method is then used for analysis of formulation. The developed method was validated in terms of ICH guidelines Q2(R1). The validation of proposed method was verified by recovery studies and was found to be satisfactory.
"Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 1, page no.a871-a882, January-2023, Available :http://www.ijnrd.org/papers/IJNRD2301098.pdf
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ISSN:
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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