Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma

Registration ID: IJNRD_185889

Published ID: IJNRD2301098

DOI: http://doi.one/10.1729/Journal.32654

R.N.Sathyaprakash , S.Ravichandran

UV, RP-HPLC, pregablin, duloxetine HCl, method development, validation.

This work is concerned with a new, simple, rapid, and precise UV and RP-HPLC method for determination of pregabalin and duloxetine hydrochloride in both bulk and in capsule. UV determination was carried out using Acetonitrile: water as solvent system. The maximum absorbance was observed at 267nm and 218nm for pregabalin and duloxetine hydrochloride respectively. Chromatographic separation was achieved isocratically using C18 column (250×4.6 mm, 5µm), mobile phase containing Acetonitrile and phosphate buffer (pH 6.8) in the ratio 40:60 maintained at flow rate 1 ml/min. Detection wavelength at 240nm. Retention time of pregabalin was found to be 1.8min and for duloxetine HCl 3.4 min. This method is then used for analysis of formulation. The developed method was validated in terms of ICH guidelines Q2(R1). The validation of proposed method was verified by recovery studies and was found to be satisfactory.

Published Paper   E-Certificate

R.N.Sathyaprakash & S.Ravichandran (January-2023). Method development and validation of pregabalin and duloxetine hydrochloride in bulk and pharmaceutical dosage form by UV and RP-HPLC using human plasma. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(1), a871-a882. http://doi.one/10.1729/Journal.32654

Volume 8 Issue 1, January-2023

Pages : a871-a882

Paper Reg. ID: IJNRD_185889

Published Paper Id: IJNRD2301098

Downloads: 000121996

Research Area: Pharmacy

Country: Chengalpattu, Tamilnadu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2301098.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2301098

Crossref DOI: http://doi.one/10.1729/Journal.32654

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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