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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | Isolation and characterization of urease producing bacteria from different soil types. |
| Authors Name: | P.S.Rayate , Dr.S.S.Ghumare |
| Download E-Certificate: | Download |
| Author Reg. ID: |
IJNRD_185433
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| Published Paper Id: | IJNRD2212296 |
| Published In: | Volume 7 Issue 12, December-2022 |
| DOI: | |
| Abstract: | Urea is hydrolyzed by means of urease enzyme with production of ammonia and carbonic acid. It provides an alkaline pH, and leads to CaCO3precipitation.This are one of the mechanism for microbially induced calcium carbonate precipitation (MICP).For this study, ureolytic bacteria capable of precipitating calcium carbonate were isolated from different types of soil samples and were tested for their urease activity. Each isolate were identified morphologically and using basic biochemical tests.Isolates were also further tested for their ability to grow in extreme environment such as high pH, high salt concentration and low nutrient availability.16 out of 28 isolates showed positive results for urease production. Hydrolysis of urea was examined using Christensen’s urea agar base. Conductivity method was used to assay urease activity and it was in the range of 0.009-0.051 mS/min.Calcium carbonate precipitation via urea hydrolysis was investigated in both solid and broth media. CaCO3crystals in the precipitation agar media were imaged.The present experimental approach can be potentially useful for a variety of environmental bioremediation applications such as heavy metal removal, self-healing concrete etc. |
| Keywords: | Urease, calcium carbonate precipitation, electric conductivity, soil bacteria. |
| Cite Article: | "Isolation and characterization of urease producing bacteria from different soil types.", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 12, page no.c984-c991, December-2022, Available :http://www.ijnrd.org/papers/IJNRD2212296.pdf |
| Downloads: | 000118754 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRD2212296 Registration ID: 185433 Published In: Volume 7 Issue 12, December-2022 DOI (Digital Object Identifier): Page No: c984-c991 Country: Nashik, Maharastra, India Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2212296 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2212296 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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