Paper Title
Isolation and characterization of urease producing bacteria from different soil types.
Article Identifiers
Authors
P.S.Rayate , Dr.S.S.Ghumare
Keywords
Urease, calcium carbonate precipitation, electric conductivity, soil bacteria.
Abstract
Urea is hydrolyzed by means of urease enzyme with production of ammonia and carbonic acid. It provides an alkaline pH, and leads to CaCO3precipitation.This are one of the mechanism for microbially induced calcium carbonate precipitation (MICP).For this study, ureolytic bacteria capable of precipitating calcium carbonate were isolated from different types of soil samples and were tested for their urease activity. Each isolate were identified morphologically and using basic biochemical tests.Isolates were also further tested for their ability to grow in extreme environment such as high pH, high salt concentration and low nutrient availability.16 out of 28 isolates showed positive results for urease production. Hydrolysis of urea was examined using Christensen’s urea agar base. Conductivity method was used to assay urease activity and it was in the range of 0.009-0.051 mS/min.Calcium carbonate precipitation via urea hydrolysis was investigated in both solid and broth media. CaCO3crystals in the precipitation agar media were imaged.The present experimental approach can be potentially useful for a variety of environmental bioremediation applications such as heavy metal removal, self-healing concrete etc.
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How To Cite (APA)
P.S.Rayate & Dr.S.S.Ghumare (December-2022). Isolation and characterization of urease producing bacteria from different soil types.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(12), c984-c991. https://ijnrd.org/papers/IJNRD2212296.pdf
Issue
Volume 7 Issue 12, December-2022
Pages : c984-c991
Other Publication Details
Paper Reg. ID: IJNRD_185433
Published Paper Id: IJNRD2212296
Downloads: 000121986
Research Area: Biological Science
Country: Nashik, Maharastra, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2212296.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2212296
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