PRODUCTION, EXTRACTION AND CHARACTERIZATION OF BIOSURFACTANT PRODUCED BY MARINE MICROBIAL ISOLATES

Registration ID: IJNRD_183106

Published ID: IJNRD2209141

: http://doi.one/10.1729/Journal.32038

Sloka K. Desai, Vrutika C. Tailor, Dr. Krishpa B. Shah

Biosurfactant producing microbes, Haemolytic activity, Triphenyl tetrazolium chloride, Bacterial Adhesion to Hydrocarbon (BATH) assay, organic solvent (chloroform- methanol), Antibacterial activity

Biosurfactants are extracellular surface active compounds produced by bacteria, fungi and yeast. Most microbial surfactants are complex molecules, comprising different structures that include lipopeptides, glycolipids, polysaccharides protein complexes, fatty acids and phospholipids. The main criteria used in this study were isolation of biosurfactants producer were hemolytic assay, bacterial adherence to hydrocarbons (BATH), visualization of bacteria in oil droplets, drop collapse test and emulsification assay. Isolation and screening of biosurfactant producing bacteria were performed from marine water and sand samples collected from Tithal beach of Valsad district and Dumas beach of Surat District, Gujarat, India and isolation was done on nutrient agar plates and Sabouraud’s agar plates, from which 9 bacterial isolates and 4 fungal isolates were obtained. Isolates were characterized and analyzed, based on different screening methods like growth on mineral salt agar medium, hemolytic assay, BATH assay, visualization of bacteria in oil droplets, drop collapse test and emulsification assay. 7 isolates were able to grow on MSM. 8β (hemolytic) and 5ɣ (non- hemolytic) isolates were obtained on blood agar plates. In BATH assay from 9 isolates, 2 isolates shown high affinity with engine oil. 6 isolates had shown the red dot of TTC absorption in visualization of bacteria in oil droplates. In drop collapse test only 1 isolate using supernatant had shown great activity. In emulsification assay only 1 isolate was found promising biosurfactant producer and is selected based on its highest emulsification activity and is used for production and extraction process. Production of biosurfactant was carried out by single isolate which one is showing high zone as well as passing all the screening test. Finally production of biosurfactants was carried out by only 1 isolate. The biosurfactant was extracted from the cell- free supernatant by centrifugation. Antibacterial activity of biosurfactant was done against the test organisms and zone of inhibition was observed. Also the qualitative analysis of the extracted biosurfactant was done by TLC and FTIR to check presence of possible functional group.

Published Paper   E-Certificate

Sloka K. Desai, Vrutika C. Tailor, & Dr. Krishpa B. Shah (September-2022). PRODUCTION, EXTRACTION AND CHARACTERIZATION OF BIOSURFACTANT PRODUCED BY MARINE MICROBIAL ISOLATES. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(9), 1199-1212. http://doi.one/10.1729/Journal.32038

Volume 7 Issue 9, September-2022

Pages : 1199-1212

Paper Reg. ID: IJNRD_183106

Published Paper Id: IJNRD2209141

Downloads: 000122254

Research Area: Science

Author Type: Indian Author

Country: Valsad, Gujarat, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2209141.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2209141

Crossref DOI: http://doi.one/10.1729/Journal.32038

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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