INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
Probiotics are one such kind of bacteria that are ingested as a supplementary food to maintain or restore beneficial bacteria of the digestive tract which has a significant impact on living being and improves the gastrointestinal microflora inside the body. This research work assessed the properties of probiotic bacteria through isolation, biochemical analysis and the antimicrobial efficacy of isolated probiotic bacteria. To conduct the research, yogurt was considered as test samples and probiotic bacteria was isolated from these samples from different Divisions (Dhaka, Chattagram, Cumilla, Khulna, Sylhet, Mymensingh and Rajshahi) of Bangladesh. Morphological, biochemical and physiological analysis showed rod shaped morphology by colony morphology observation, Gram positive properties by Gram staining technique, non-motile properties by motility test and coagulase positive and catalase negative properties by coagulase test and catalase test respectively. These properties facilitated the identification of probiotic bacteria. To identify the presumptive probiotics, sugar fermentation pattern, 2-6% NaCl test, 0.4% phenol tolerance level test, 0.3% bile tolerance level test and pH resistance test were considered in which the probiotics showed excellent growth. Antimicrobial activity was observed using isolated bacteria against eight enteric pathogens. For determining the antimicrobial efficacy, pathogen-induced mice model trail was conducted by feeding with probiotic yogurt at different concentrations. At the end of the fifth week of probiotic treatment, the 3rd Treatment group (150 ml yogurt per kg body weight) showed significant (p<0.001) antimicrobial activity. In comparison to 2nd Treatment group (100 ml yogurt per kg body weight) and 3rd Treatment group (150 ml yogurt per kg body weight) with Control group, the mean difference was found significant (p<0.001) for the growth of inhibition zone of pathogenic bacteria. No significant difference was observed while the Standard group was compared to 3rd Treatment group especially for the measurement of IgG level. However, 3rd Treatment group exhibited significant (p<0.001) difference than the 2nd Treatment group and Control group. In the case of the body weight measurement, when the 3rd Treatment group was compared with Control group, the 3rd Treatment group gained a significant (p<0.001) body weight. In case of white blood cell count and determination of total protein level, albumin level and hemoglobin level, the treatment groups showed significant value than Control group. Molecular identification of isolated probiotic bacteria would be worthy to investigate for further development of probiotic product.
"THE EFFECTS OF PROBIOTIC ISOLATES AGAINST ENTERIC PATHOGENIC MICROORGANISMS IN MICE MODEL", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 8, page no.906-925, August-2022, Available :http://www.ijnrd.org/papers/IJNRD2208097.pdf
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2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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