THE EFFECTS OF PROBIOTIC ISOLATES AGAINST ENTERIC PATHOGENIC MICROORGANISMS IN MICE MODEL

THE EFFECTS OF PROBIOTIC ISOLATES AGAINST ENTERIC PATHOGENIC MICROORGANISMS IN MICE MODEL

Registration ID: IJNRD_182438

Published ID: IJNRD2208097

DOI: Click Here to Get

Antara Sarkar , Khondoker Moazzem Hossain , S. M. Mahbubur Rahman , Amit Sarder

Probiotic, Antimicrobial Activity, Mice Model, Pathogen, Lactic Acid Bacteria

Probiotics are one such kind of bacteria that are ingested as a supplementary food to maintain or restore beneficial bacteria of the digestive tract which has a significant impact on living being and improves the gastrointestinal microflora inside the body. This research work assessed the properties of probiotic bacteria through isolation, biochemical analysis and the antimicrobial efficacy of isolated probiotic bacteria. To conduct the research, yogurt was considered as test samples and probiotic bacteria was isolated from these samples from different Divisions (Dhaka, Chattagram, Cumilla, Khulna, Sylhet, Mymensingh and Rajshahi) of Bangladesh. Morphological, biochemical and physiological analysis showed rod shaped morphology by colony morphology observation, Gram positive properties by Gram staining technique, non-motile properties by motility test and coagulase positive and catalase negative properties by coagulase test and catalase test respectively. These properties facilitated the identification of probiotic bacteria. To identify the presumptive probiotics, sugar fermentation pattern, 2-6% NaCl test, 0.4% phenol tolerance level test, 0.3% bile tolerance level test and pH resistance test were considered in which the probiotics showed excellent growth. Antimicrobial activity was observed using isolated bacteria against eight enteric pathogens. For determining the antimicrobial efficacy, pathogen-induced mice model trail was conducted by feeding with probiotic yogurt at different concentrations. At the end of the fifth week of probiotic treatment, the 3rd Treatment group (150 ml yogurt per kg body weight) showed significant (p<0.001) antimicrobial activity. In comparison to 2nd Treatment group (100 ml yogurt per kg body weight) and 3rd Treatment group (150 ml yogurt per kg body weight) with Control group, the mean difference was found significant (p<0.001) for the growth of inhibition zone of pathogenic bacteria. No significant difference was observed while the Standard group was compared to 3rd Treatment group especially for the measurement of IgG level. However, 3rd Treatment group exhibited significant (p<0.001) difference than the 2nd Treatment group and Control group. In the case of the body weight measurement, when the 3rd Treatment group was compared with Control group, the 3rd Treatment group gained a significant (p<0.001) body weight. In case of white blood cell count and determination of total protein level, albumin level and hemoglobin level, the treatment groups showed significant value than Control group. Molecular identification of isolated probiotic bacteria would be worthy to investigate for further development of probiotic product.

Published Paper   E-Certificate

"THE EFFECTS OF PROBIOTIC ISOLATES AGAINST ENTERIC PATHOGENIC MICROORGANISMS IN MICE MODEL", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.7, Issue 8, page no.906-925, August-2022, Available :https://ijnrd.org/papers/IJNRD2208097.pdf

Volume 7 Issue 8, August-2022

Pages : 906-925

Paper Reg. ID: IJNRD_182438

Published Paper Id: IJNRD2208097

Downloads: 00048

Research Area: Life Sciences

Country: Khulna, Khulna, Bangladesh

Published Paper PDF: https://ijnrd.org/papers/IJNRD2208097.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2208097

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

Call For Paper - Volume 10 | Issue 8 | August 2025

IJNRD is Scholarly open access journals, Peer-reviewed, and Refereed Journals, High Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool), Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) with Open-Access Publications.

INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to explore advances in research pertaining to applied, theoretical and experimental Technological studies. The goal is to promote scientific information interchange between researchers, developers, engineers, students, and practitioners working in and around the world. IJNRD will provide an opportunity for practitioners and educators of engineering field to exchange research evidence, models of best practice and innovative ideas.

Indexing In Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar | AI-Powered Research Tool, Microsoft Academic, Academia.edu, arXiv.org, Research Gate, CiteSeerX, ResearcherID Thomson Reuters, Mendeley : reference manager, DocStoc, ISSUU, Scribd, and many more

How to submit the paper?

Important Dates for Current issue

Paper Submission Open For: August 2025

Current Issue: Volume 10 | Issue 8

Last Date for Paper Submission: Till 31-Aug-2025

Notification of Review Result: Within 1-2 Days after Submitting paper.

Publication of Paper: Within 01-02 Days after Submititng documents.

Frequency: Monthly (12 issue Annually).

Journal Type: International Peer-reviewed, Refereed, and Open Access Journal.

Subject Category: Research Area