INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
An accurate, precise, economical, reproducible high-performance liquid Chromatographic method has been developed for the quantitative estimation of Metformin hydrochloride and Linagliptin simultaneously in the tablet dosage form. The instruments consisted of Agilent model G1310B 1260 infinity Iso pump equipped with a UV detector and Agilent model 1260 infinity autosampler equipped with a UV detector. The separation was carried out using Agilent column C18 150 cm 25 cm x 4.6 mm packed with Octadecylsilane bonded to porous silica (5µm) with a flow rate of 1.0 ml/min, column temperature ambient, and UV detector set at 265 and 295 nm. The mobile phase is comprised of phosphate Buffer 0.02M (2.7216 g Potassium Dihydrogen Orthophosphate dissolved in 1000 ml of water and, Acetonitrile in the ratio of 70:30 (v/v). The method was selective for Metformin HCL and Linagliptin with retention times 0.954 min and 2.226 min, respectively. The linearity was established over the 4000-6000 mcg/ml concentration range and 20-30 mcg/ml for Metformin HCL and Linagliptin, respectively. The developed method was validated by ICH harmonized tripartite guidelines: “Validation of analytical procedures: text and methodology Q2(R1)”. The statistical result showed that the method was precise, accurate, reproducible and specific for the analysis of Metformin HCL and Linagliptin.
The marketed sample of Metformin HCL and Linagliptin combination immediate release tablet dosage form was analysed by the developed method. The proposed method is suitable for routine quality control analysis of Metformin Hydrochloride and Linagliptin in a Tablet dosage form.
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"ANALYTICAL METHOD VALIDATION OF METFORMIN HCL AND LINAGLIPTIN TABLET BY HPLC", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 8, page no.516-524, August-2022, Available :http://www.ijnrd.org/papers/IJNRD2208058.pdf
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2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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