Paper Title
Analytical Method Development and Validation for Simultaneous estimation of Metformin Hydrochloride and Empagliflozin by RP-HPLC in Bulk and it’s Tablet dosage form
Article Identifiers
Authors
Nutan Rajendra Chapaitkar , Dr. D.V. Derle
Keywords
Metformin Hydrochloride Empagliflozin, Method Development, Validation, HPLC, Linearity, LOD, LOQ, Precision.
Abstract
Metformin is a class of drug called Biguanides. It is used to control high blood sugar in type 2 diabetes ,hence it help to prevent kidney damage, blindness, nerve damage, sexual problems, while in case of Empagliflozin it is belong to class sodium glucose co- transporter SGLT-2 inhibitor it lowers blood sugar by causing kidney to get rid of more glucose in the urine. The present work is based on excludation of buffer solution in mobile phase and use of cheap solvent in development of cost effective method. Result: The Chromatographic separation was achieved on Chemsil C18 column used as stationary phase and Methanol : Water (65:35% v/v) used as mobile phase. The flow rate was 1ml/min and the absorbance was monitored at 227nm. Metformin Hydrochloride and Empagliflozin was eluted at 2.273min and 4.117min. The procedure and capabilities were evaluated against system suitability, linearity, precision ,accuracy, LOD,LOQ and Robustness as per ICH Q2 (R1) guidelines. The method shows good linearity ( R2=0.9992 and 0.9996)
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How To Cite (APA)
Nutan Rajendra Chapaitkar & Dr. D.V. Derle (August-2022). Analytical Method Development and Validation for Simultaneous estimation of Metformin Hydrochloride and Empagliflozin by RP-HPLC in Bulk and it’s Tablet dosage form. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(8), 340-355. https://ijnrd.org/papers/IJNRD2208039.pdf
Issue
Volume 7 Issue 8, August-2022
Pages : 340-355
Other Publication Details
Paper Reg. ID: IJNRD_182378
Published Paper Id: IJNRD2208039
Downloads: 000121999
Research Area: Pharmacy
Country: Aurangabad, Maharastra, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2208039.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2208039
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition


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