Paper Title
Genetic Diversity among sixteen different Actinomycetes species isolated from Lonar Crater using ITS marker
Article Identifiers
Authors
S. N. Mendhe , S. N. Mendhe , A. M. Garode
Keywords
Actinomycetes; Lonar lake; ITS, molecular; Phylogenetic study; Streptomyces.
Abstract
Isolation studies of natural and ecologically enriched units help in achieving novel secondary metabolites rich in actinomycete species. The Lonar lake crater of the Buldhana district is one of the less explored naturally occurring alkaline ecosystems for the screening and discovery of a unique microbial community. The capability of marine actinomycetes to survive in extreme temperature and pH conditions enhances their bioactive system for the secretion of novel secondary metabolites. In the present study, we performed a study of sixteen- isolates of actinomycetes species isolated from Lonar lake, Buldhana District, Maharashtra. The isolation and the identification of the isolates were performed through molecular tools and phylogenetic analysis. . The phylogenetic relationship was determined by r-DNA-based phylogenetic markers ITS (Internal Transcribed Spacers) with the help of phylogenetic tools. The present study aimed to assess genetic variations among sixteen- isolates of actinomycetes isolated from Lonar lake by using ITS molecular marker. The dendrogram obtained from the data showed that hierarchical clustering separated the isolates into two groups. The results showed that the majority of the isolates belonged to Streptomyces species, 2 isolates of Brevibacterium species and single isolates of Kocuria, Conexibacter, Rubrobacter, Actinomadura, Micromonospora, Nocardiopsis, and Actinobiospora. ITS can be used as an important molecular marker for phylogenetic study.
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How To Cite (APA)
S. N. Mendhe, S. N. Mendhe, & A. M. Garode (August-2022). Genetic Diversity among sixteen different Actinomycetes species isolated from Lonar Crater using ITS marker. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(8), 50-55. https://ijnrd.org/papers/IJNRD2208007.pdf
Issue
Volume 7 Issue 8, August-2022
Pages : 50-55
Other Publication Details
Paper Reg. ID: IJNRD_182325
Published Paper Id: IJNRD2208007
Downloads: 000121983
Research Area: Biological Science
Country: Buldana, Maharashtra, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2208007.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2208007
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