INCREASING PHYTO-CHEMICAL EXPRESSIONS WITH ENZYME INFUSION

INCREASING PHYTO-CHEMICAL EXPRESSIONS WITH ENZYME INFUSION

Registration ID: IJNRD_182245

Published ID: IJNRD2207149

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Emoleila Itoandon , Femi Adams , Salau Adetola Moisat , Muhammad Akram

Biocatalyst, Hydrolysis, Plant Microbial Toxicity, Pharmacognosis.

An investigation was carried out to determine effect of enzyme infusion on concentration of phytochemicals for enhanced antimicrobial expression. To achieve the aim: i. microbial alpha amylase and cellulase were synthesized using solid state fermentation technique, ii. powdery sample of Combretodendrum microcarpon was processed and infused with a consortium of synthases, and iii. infused extract was concentrated and analysed for phyto-inhibitory expressions. The results obtained showed 10% diluted enzymes recorded optimal activities of 3.62 U/ml and 3.23 U/ml at 50oC and pH 7.0 for alpha amylase while cellulase had activities of 3.54 U/ml and 2.98 U/ml at 60oC and pH 5.0 respectively. Effect of enzyme infusion on powdery sample recorded a 45% increase in concentration of identified bioactive compounds (alkaloids, flavonoids, steroids, terpenoids, anthraquinone) which was observed in the phyto-inhibition expressions for Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Enterobacter aerogenes respectively against controls. The investigation has furthermore proven the importance and relevance of enzyme application in discovery of new compounds for drug discovery.

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"INCREASING PHYTO-CHEMICAL EXPRESSIONS WITH ENZYME INFUSION ", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.7, Issue 7, page no.1338-1347, July-2022, Available :https://ijnrd.org/papers/IJNRD2207149.pdf

Volume 7 Issue 7, July-2022

Pages : 1338-1347

Paper Reg. ID: IJNRD_182245

Published Paper Id: IJNRD2207149

Downloads: 000121112

Research Area: Science

Country: Chennai, Tamil Nadu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2207149.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2207149

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

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