Production and Optimization of Polyhydroxy butyrate-(PHB) from Bacterial isolates.

Registration ID: IJNRD_182186

Published ID: IJNRD2207132

: http://doi.one/10.1729/Journal.31118

Rajvi Patel, Nirali Patel, Hetal Panchal

Bioplastic, Polyhydroxybutyrate-(PHB), Optimization, FTIR spectroscopy

PHBs are storage material synthesize by different microorganisms.The present study was focused on Production of PHB by bacterial isolates isolated from local soil samples.All the bacterial isolates were screened for their PHB producing ability and two isolates showing highest PHB production were selected for further studies.Optimization of different variables like pH, temperature, incubation period, carbon and nitrogen sources were performed.The maximum PHB accumulation was found when the medium was supplemented with glucose and sucrose as a carbon source. Yeast extract and peptone were found optimum nitrogen source.The optimum pH and temperature were 6.5-7.0 and 35°C respectively. The polymer can be further purified by dissolving in chloroform and precipitating with cold ethanol. PHB extraction carried out using sodium hypochlorite method exhibited a good PHB yield amounting to 73% and 80% for S1b and S3b isolates respectively. The extracted compounds on FTIR analysis gave characteristics functional units as C-H, CH2 ,-OH,C=O groups. UV-Vis spectrophotometric analysis provide confirmed similarity of extracted PHB and its suitability for bioplastic production.

Published Paper   E-Certificate

Rajvi Patel, Nirali Patel, & Hetal Panchal (July-2022). Production and Optimization of Polyhydroxy butyrate-(PHB) from Bacterial isolates.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(7), 1148-1157. http://doi.one/10.1729/Journal.31118

Volume 7 Issue 7, July-2022

Pages : 1148-1157

Paper Reg. ID: IJNRD_182186

Published Paper Id: IJNRD2207132

Downloads: 000122256

Research Area: Biological Science

Author Type: Indian Author

Country: Valsad, Gujrat, India

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Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2207132

Crossref DOI: http://doi.one/10.1729/Journal.31118

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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