Paper Title
In vitro intensification of anthraquinones in callus cultures of Rubia cordifolia by precursor feeding
Article Identifiers
Authors
Dr. Devi Priya M. , Dr. EA Siril
Keywords
Alizarin, purpurin, anthraquinone, phenylalanine, ketoglutarate
Abstract
Background: Plants are enriched with various secondary metabolites. It’s over exploitation may undermine the existence of plants in this world. So plant tissue culture technology is now emerging as a new frontier in science, as it helps to improve the production rate of metabolites in in vitro condition. Callus cultures were developed on different auxin supplemented media and the callus were maintained at the optimum concentration. Precursor feeding experiment were conducted to find the increment of AQ production under the influence of precursors used viz., ketoglutarate (KG) and phenylalanine (PA). Friable calli were produced from nodal segments cultured on MS medium containing 5 µM NAA and estimated 17.76 mg g-1 dw alizarin and 3.8 mg g-1 dw purpurin. In callus cultures addition 100 µM phenylalanine as precursor has resulted 25 fold higher alizarin production over roots sampled from naturally growing plant. This work is focused to improve the AQ production through callus culture of R. cordifolia so that we could maintain the continuous supply of AQ without disturbing the plant intact.
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How To Cite (APA)
Dr. Devi Priya M. & Dr. EA Siril (June-2022). In vitro intensification of anthraquinones in callus cultures of Rubia cordifolia by precursor feeding. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(6), 369-375. https://ijnrd.org/papers/IJNRD2206041.pdf
Issue
Volume 7 Issue 6, June-2022
Pages : 369-375
Other Publication Details
Paper Reg. ID: IJNRD_181740
Published Paper Id: IJNRD2206041
Downloads: 000121984
Research Area: Science & Technology
Country: Pathanamthitta, Kerala, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2206041.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2206041
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