Registration ID: IJNRD_181395

Published ID: IJNRD2205071

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THALLA VENKATA SIVA LAKSHMI , DR. KEESAMSETTY SARALA , DR. KUDUPUDI PRABHAKARA RAO , ALAVALA MATTA REDDY , T. G. K. MURTHY

Key words: sps : Solanesyl-di-phosphate syn-thase, pP2A protein phosphatase 2A, HEAT : Huntingtin. Elongation Factor3 (EF3), Pro-tein phosphotase2A (pP2A) and Yeast Kinase TOR1), HDBRG : Harvel De Bouxo Rio Grande,

ABSTRACT Solanesol (C45H74O), a tri-sesqui-terpene al-cohol is a derivative of Solanesyl-di-phosphate but the key enzyme involved in the catalysis is unknown. So, a study was under-taken to explore the enzyme behind the value addition. Burley variety HDBRG. a high solanesol line and a Flue-cured variety Siri, a low solanesol line of tobacco were selected for the experi-mental and exploration studies from Experi-mental Farm of Central Tobacco Research Institute at Katheru. mRNA was isolated from top leaves, middle leaves; stem and roots as Solanesol has its presence both as free and esterified forms. Key Genes involved in Solanesol biosynthetic pathway are dxs (1-deoxy-D-xylulose-5-phosphate synthase), dxr (1-de-oxy-D-xylulose-5-phosphate reductase), sps (Solanesyl-di-phosphate synthase) and pP2A (protein phosphatase 2A). Primers were designed. Taking Plastid encoded 16SrRNA as normalising gene. Qiagen RT-PCR was done. 16S rRNA is found to express uni-formly in both the lines across the samples. Studies revealed gene sps has the maximum gene expression followed by genes pp2A, dxs and dxr in solanesol biosynthesis. Since the gene expression is inversely propor-tional to CT values gene sps having minimum CT value exhibit higher expression relative to other genes viz, pp2A. dxs, dxr. pP2A of MPP super family having metallo-phosphatase domain remarkable for HEAT repeats is playing a vital role in differentiating High Solanesol RIL, HDBRG and Low Solanesol Line, Siri. It is thought to be the potential enzyme involved in the conversion of Solanesyl-di-phosphate to Solanesol

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"", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.7, Issue 5, page no.641-652, May-2022, Available :https://ijnrd.org/papers/IJNRD2205071.pdf

Volume 7 Issue 5, May-2022

Pages : 641-652

Paper Reg. ID: IJNRD_181395

Published Paper Id: IJNRD2205071

Downloads: 000121179

Research Area: Pharmacy

Country: HUKKUMPET, RAJAMAHENDRAVARAM,ANDHRA PRADESH, INDIA, INDIA

Published Paper PDF: https://ijnrd.org/papers/IJNRD2205071.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2205071

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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