Invitro Regeneration from Stem node explants of Celestrus paniculatrus(Wild)

Registration ID: IJNRD_180427

Published ID: IJNRD2201019

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Mandaloju Venkateshwarlu

Key words: Regeneration Stem node, BAP, Kn, L-Glutamic acid, Celestrus paniculatrus

Code:60/2022/1 Invitro Regeneration from Stem node explants of Celestrus paniculatrus(Wild) Mandaloju Venkateshwarlu MSc.,Ph.D.,Botany,MSc.,Biotechnology,FHAS,SFS,SFAB,CFE and LLB Department of Botany, Kakatiya University, Warangal Urabn – 506 009, T.S. INDIA Email: drvenkat6666@gmail.com. ABSTRACT The primary aim of this study have been to gain some knowledge about the genotypic differnces for callus induction and high frequency plant regeneration stem node segments of Celestrus paniculatrus were inoculated on MS basal medium supplemented with various cytokines BAP and NAA Coconut water also had a role in triggering the formation of multiple shoots. Addition of BAP at 2.0 mg/l and NAA at 3.0 mg/l to the MS basal medium, induced regeneration from Stem node expalnts with an increase in the level of BAP 2.0 – 4.0 mg/l the percentage of explants producing shoots also increased. The number of shoots developed on the Stem node expalnts ranged from 1-4 to 2-3 by the addition of BAP at concentration of 2.0 mg/l or NAA at 3.0 mg/l. Among the three concentrations of coconut milk used i.e., 10,15 and 20% , 15% of coconut milk along with 0.5 mg/l BAP + Kn proved to be ideal for multiple shoot induction. MS medium fortified with 1.0 mg/l BAP or 2.0 mg/l L-G Glutamic acid also induced shoot buds on Stem node explants. Plant tissue culture and callus induction from cotyledonary segments,M.Venkateshwarlu(2021).

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Mandaloju Venkateshwarlu (January-2022). Invitro Regeneration from Stem node explants of Celestrus paniculatrus(Wild). INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(1), 125-128. https://ijnrd.org/papers/IJNRD2201019.pdf

Volume 7 Issue 1, January-2022

Pages : 125-128

Paper Reg. ID: IJNRD_180427

Published Paper Id: IJNRD2201019

Downloads: 000122254

Research Area: Life Sciences 

Author Type: Indian Author

Country: Warangal , Telangana, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2201019.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2201019

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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