Paper Title
SIROLIMUS: PRODUCTION ENHANCEMENT USING MUTAGENESIS AND MEDIUM OPTIMISATION
Article Identifiers
Authors
Prabhakar Babu , Dr. Umesh Luthra , Ilma Majeed , Siva Sankari
Keywords
fermentation, optimization, Plackett-Burman design, OVAT, Streptomyces hygroscopicus, UV, NTG
Abstract
Sirolimus (Rapamycin) is a coveted drug substance obtained from actinobacteria named Streptomyces hygroscopicus, which synthesizes it as a secondary metabolite. It has been studied in regard to a variety of biological conditions such as anti-pathogenicity, immunosuppression, anti-aging, and anti-cancerous activity. Devising methodologies to obtain a sufficient amount of sirolimus is a part of eminent research prospects. For the current study, enhancement in sirolimus production has been achieved by following a sequential regime involving improvement in the fermentation media and mutation of the parent strain along with careful and continuous screening at each step. The investigation encompasses the establishment of initial production activity followed by optimization of medium components using Plackett-Burman design and OVAT methodology. The significant components obtained have been thoroughly monitored to finalize the concentration which yielded maximum production of sirolimus. Then the procured strain of Streptomyces hygroscopicus has been mutated using intermittent treatment with UV radiations. The stable culture colonies obtained thereby have been taken for chemical mutagenesis using NTG. The colonies offering stable and enhanced yield have been isolated to inoculate the optimized media. Finally, 2.5 to 2.8 - fold augmentation in the production of sirolimus has been observed concerning the initially recorded activity.
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How To Cite (APA)
Prabhakar Babu, Dr. Umesh Luthra, Ilma Majeed, & Siva Sankari (January-2022). SIROLIMUS: PRODUCTION ENHANCEMENT USING MUTAGENESIS AND MEDIUM OPTIMISATION. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 7(1), 100-108. https://ijnrd.org/papers/IJNRD2201015.pdf
Issue
Volume 7 Issue 1, January-2022
Pages : 100-108
Other Publication Details
Paper Reg. ID: IJNRD_180417
Published Paper Id: IJNRD2201015
Downloads: 000121975
Research Area: Science & Technology
Country: PONDICHERRY, PONDICHERRY, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2201015.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2201015
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
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