Paper Title
Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin and Irbesartan for combined dosage form
Article Identifiers
Authors
Vijaya M Gaikwad , Vivekanand A Kashid , Kiran B Kotade
Keywords
Irbesartan, Atorvastatin, RP-HPLC, UV, Validation
Abstract
ABSTRACT A specific, precise and accurate RP-HPLC method has been developed for simultaneous estimation of irbesartan and atorvastatin in synthetic mixture. The use of a 250 × 4.6 mm, 5 µm particle size, C18 column with 50: 10: 40 %, v/v/v acetonitrile: methanol: 0.1 % formic acid (pH adjusted to 3.5 with triethylamine) as isocratic mobile phase at flow rate of 1 ml/min enabled separation of drugs. UV detection was performed at 271 nm. The method was validated as per ICH Q2(R1) guidelines. The retention time for irbesartan and atorvastatin were found to be 4.01 ± 0.03 and 7.03 ± 0.03 min respectively. The linearity of method was satisfactory over the range 300-1500 ng (correlation coefficient 0.9993) and 20-100 ng (correlation coefficient 0.9996) for irbesartan and atorvastatin respectively. The limits of detection for irbesartan and atorvastatin were 10.94 and 1.33 ng respectively. The limits of quantitation for irbesartan and atorvastatin were 33.14 and 4.03 ng respectively. Recovery of irbesartan and atorvastatin ranged from 99.84-99.98 % and 99.90-100.67 % respectively. The method was successfully applied to synthetic mixture for quantitative analysis of irbesartan and atorvastatin. Keyword- Irbesartan, Atorvastatin, RP-HPLC, analysis
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How To Cite (APA)
Vijaya M Gaikwad, Vivekanand A Kashid, & Kiran B Kotade (November-2020). Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin and Irbesartan for combined dosage form. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 5(11), 347-391. https://ijnrd.org/papers/IJNRD2011039.pdf
Issue
Volume 5 Issue 11, November-2020
Pages : 347-391
Other Publication Details
Paper Reg. ID: IJNRD_180338
Published Paper Id: IJNRD2011039
Downloads: 000121988
Research Area: Pharmacy
Country: Nashik, Maharashtra, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2011039.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2011039
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Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition
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