Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin and Irbesartan for combined dosage form

Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin and Irbesartan for combined dosage form

Registration ID: IJNRD_180338

Published ID: IJNRD2011039

DOI: Click Here to Get

Vijaya M Gaikwad , Vivekanand A Kashid , Kiran B Kotade

Irbesartan, Atorvastatin, RP-HPLC, UV, Validation

ABSTRACT A specific, precise and accurate RP-HPLC method has been developed for simultaneous estimation of irbesartan and atorvastatin in synthetic mixture. The use of a 250 × 4.6 mm, 5 µm particle size, C18 column with 50: 10: 40 %, v/v/v acetonitrile: methanol: 0.1 % formic acid (pH adjusted to 3.5 with triethylamine) as isocratic mobile phase at flow rate of 1 ml/min enabled separation of drugs. UV detection was performed at 271 nm. The method was validated as per ICH Q2(R1) guidelines. The retention time for irbesartan and atorvastatin were found to be 4.01 ± 0.03 and 7.03 ± 0.03 min respectively. The linearity of method was satisfactory over the range 300-1500 ng (correlation coefficient 0.9993) and 20-100 ng (correlation coefficient 0.9996) for irbesartan and atorvastatin respectively. The limits of detection for irbesartan and atorvastatin were 10.94 and 1.33 ng respectively. The limits of quantitation for irbesartan and atorvastatin were 33.14 and 4.03 ng respectively. Recovery of irbesartan and atorvastatin ranged from 99.84-99.98 % and 99.90-100.67 % respectively. The method was successfully applied to synthetic mixture for quantitative analysis of irbesartan and atorvastatin. Keyword- Irbesartan, Atorvastatin, RP-HPLC, analysis

Published Paper   E-Certificate

"Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin and Irbesartan for combined dosage form", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.5, Issue 11, page no.347-391, November-2020, Available :https://ijnrd.org/papers/IJNRD2011039.pdf

Volume 5 Issue 11, November-2020

Pages : 347-391

Paper Reg. ID: IJNRD_180338

Published Paper Id: IJNRD2011039

Downloads: 000121143

Research Area: Pharmacy

Country: Nashik, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2011039.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2011039

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

Call For Paper - Volume 10 | Issue 8 | August 2025

IJNRD is Scholarly open access journals, Peer-reviewed, and Refereed Journals, High Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool), Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) with Open-Access Publications.

INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to explore advances in research pertaining to applied, theoretical and experimental Technological studies. The goal is to promote scientific information interchange between researchers, developers, engineers, students, and practitioners working in and around the world. IJNRD will provide an opportunity for practitioners and educators of engineering field to exchange research evidence, models of best practice and innovative ideas.

Indexing In Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar | AI-Powered Research Tool, Microsoft Academic, Academia.edu, arXiv.org, Research Gate, CiteSeerX, ResearcherID Thomson Reuters, Mendeley : reference manager, DocStoc, ISSUU, Scribd, and many more

How to submit the paper?

Important Dates for Current issue

Paper Submission Open For: August 2025

Current Issue: Volume 10 | Issue 8

Last Date for Paper Submission: Till 31-Aug-2025

Notification of Review Result: Within 1-2 Days after Submitting paper.

Publication of Paper: Within 01-02 Days after Submititng documents.

Frequency: Monthly (12 issue Annually).

Journal Type: International Peer-reviewed, Refereed, and Open Access Journal.

Subject Category: Research Area